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High resolution magic angle spinning NMR spectroscopy for metabolic assessment of cancer presence and Gleason score in human prostate needle biopsies

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Magnetic Resonance Materials in Physics, Biology and Medicine Aims and scope Submit manuscript

Abstract

Objectives

Histopathology of prostate needle biopsies (PNBs) is an important part in the diagnosis, prognosis and treatment evaluation of prostate cancer. The determination of metabolite levels in the same biopsies may have additional clinical value. Here, we demonstrate the use of non-destructive high resolution magic angle spinning (HRMAS) proton NMR Spectroscopy for the assessment of metabolic profiles of prostate tissue in PNBs as commonly obtained in standard clinical practice.

Materials and methods

PNBs that were taken routinely from 48 patients suspected of having prostate cancer were subjected to HRMAS proton NMR spectroscopy. Subsequent histopathology of the same biopsies classified the tissue either as cancer (n = 10) or benign (n = 30).

Results

Some practical aspects of this assessment were evaluated, such as typical spectral contamination caused by the PNB procedure. Significant metabolic differences were found between malignant and benign tissue using a small set of ratio’s involving signals of choline compounds, citrate and lactate. Moreover, significant correlations were observed between choline, total choline, and citrate over creatine signal ratios and the Gleason scores of tumor in PNBs and of tumor in the whole prostate.

Conclusion

This preliminary study indicates that HRMAS NMR of routinely obtained PNBs can provide detailed metabolic information of intact prostate tissue with clinical relevance.

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Authors and Affiliations

  1. Department of Radiology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands

    Jack J. A. van Asten, Claudia Soede-Huijbregts & Arend Heerschap

  2. Department of Pathology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands

    Vincent Cuijpers, Christina Hulsbergen-van de Kaa & Albert Verhofstad

  3. Department of Urology, Radboud University Nijmegen Medical Center, Nijmegen, The Netherlands

    J. Alfred Witjes

  4. Department of Radiology (430), University Medical Center Nijmegen, P.O. Box 9101, 6500 HB, Nijmegen, The Netherlands

    Arend Heerschap

Authors
  1. Jack J. A. van Asten
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  2. Vincent Cuijpers
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  3. Christina Hulsbergen-van de Kaa
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  4. Claudia Soede-Huijbregts
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  5. J. Alfred Witjes
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  6. Albert Verhofstad
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  7. Arend Heerschap
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Correspondence to Jack J. A. van Asten or Arend Heerschap.

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Albert Verhofstad died before publication of this article.

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van Asten, J.J.A., Cuijpers, V., Hulsbergen-van de Kaa, C. et al. High resolution magic angle spinning NMR spectroscopy for metabolic assessment of cancer presence and Gleason score in human prostate needle biopsies. Magn Reson Mater Phy 21, 435–442 (2008). https://doi.org/10.1007/s10334-008-0156-9

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  • DOI: https://doi.org/10.1007/s10334-008-0156-9

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