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23Na microscopy of the mouse heart in vivo using density-weighted chemical shift imaging

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Abstract

The mouse has become an important animal model for human cardiac disease, and the development of techniques for non-invasive imaging of the mouse heart in vivo is, therefore, of great potential interest. Previous magnetic resonance imaging studies have concentrated on pathologically induced changes in cardiac structure and dynamics by acquiring proton images. Further information can be gained by studying cardiac function and physiology using other nuclei, for example, sodium. Sodium imaging of such a small structure presents considerable technical challenges. In this work we show the first sodium images of the mouse heart, with an isotropic spatial resolution of 1 × 1 × 1 mm, acquired in a time of 1.5 h. The ventricles, septum and myocardium are readily distinguishable in these images, which were acquired through the combination of 3D density-weighted chemical shift imaging, optimized instrumentation, and a high magnetic field strength (17.6 T). Measurements of the myocardial:blood sodium concentration in the left and right ventricles agree well with theoretical values.

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Acknowledgments.

We would like to thank Sebastian Aussenhofer for technical support and work with AMIRA. The 17.6 T spectrometer was funded by the Deutsche Forschungsgemeinschaft (Ha1232/13). TN and AGW acknowledge financial support from the Alexander von Humboldt Foundation, Wolfgang Paul Programme.

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Authors and Affiliations

  1. Department of Physics, EP5 (Biophysics), University of Würzburg, Am Hubland, 97074, Würzburg, Germany

    T. Neuberger, A. Greiser, M. Nahrendorf, P. M. Jakob, C. Faber & A. G. Webb

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  1. T. Neuberger
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  2. A. Greiser
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  3. M. Nahrendorf
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  4. P. M. Jakob
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  5. C. Faber
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  6. A. G. Webb
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Correspondence to T. Neuberger.

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Neuberger, T., Greiser, A., Nahrendorf, M. et al. 23Na microscopy of the mouse heart in vivo using density-weighted chemical shift imaging. MAGMA 17, 196–200 (2004). https://doi.org/10.1007/s10334-004-0048-6

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  • DOI: https://doi.org/10.1007/s10334-004-0048-6

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