Abstract
Objective
The aim of the study was to investigate the differential expression of microRNAs (miRNAs) in bladder transitional cell carcinoma (BTC).
Methods
Fresh tissues were obtained from patients with BTC (9 cases; 3 cases with grade I, 3 cases with grade II, 3 cases with grade III) and those with normal bladder mucosa (3 cases) and stored in liquid nitrogen. Total RNA was extracted using TRizol reagent and RNA was quantified and quality control was performed. miRNA probes were labeled with Hy3™ fluorescence, then hybridized with a miRCURY™ array labeling kit. miRNA arrays were scanned and analyzed and the scanned result was validated using reverse transcription-polymerase chain reaction (RT-PCR).
Results
In four groups of differentially expressed genes obtained from grade I, grade II, grade III, and grade I + grade II + grade III BTC tissues compared with normal bladder mucosa, hsa-miR-29b-1* was upregulated, and hsa-miR-923 and hsa-miR-300 were downregulated. The hsa-miR-29b-1*, hsa-miR-300, and hsa-miR-923 findings were confirmed by real-time RT-PCR.
Conclusion
Genes that were differentially expressed between BTC and normal bladder mucosa may be involved in the pathogenesis and development of BTC, and may be useful for further studies of BTC-related genes.
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Supported by a grant of National Natural Science Foundation of China (No. 30772278).
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Xu, F., Wei, Z., Zhang, Z. et al. Differential expression of microRNA clusters in bladder transitional cell carcinoma. Chin. -Ger. J. Clin. Oncol. 12, 285–289 (2013). https://doi.org/10.1007/s10330-013-1138-6
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DOI: https://doi.org/10.1007/s10330-013-1138-6