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The impact of Cinobufacini injection on proliferation and apoptosis of human hepatoma HepG-2 cells

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The Chinese-German Journal of Clinical Oncology

Abstract

Objective

The aim of our study was to investigate the effect of Cinobufacini injection on the proliferation and apoptosis of human hepatocarcinoma HepG-2 cells.

Methods

Cells proliferation was assessed by MTT assay, cells morphologic was observed by the inverted microscopy, Annexin V/PI stain was used to detect the apoptosis and necrosis of the tumor cells. The expression of TOPO I mRNA and TOPO II mRNA were examined by RT-PCR.

Results

Cinobufacini injection significantly inhibited HepG-2 cells proliferation in dose- and time-dependent ways. After Cinobufacini injection intervention, HepG-2 cells showed typical morphological changes: cells changed from polygon into round, chromatin looseness and karyolysis were observed. The percentages of apoptosis were 88.49%, 76.02%, 61.73% corresponding to the 48 h interference of 0.42 μg/mL, 0.21 μg/mL, 0.105 μg/mL Cinobufacini injection, perspectively. RT-PCR assay showed that Cinobufacini injection down-regulated TOPO I and TOPO II expression at mRNA level.

Conclusion

Cinobufacini can inhibit human hepatocarcinoma HepG-2 cells growth and induce tumor cells apoptosis, the mechanism of which might partly relate to the down-regulation of TOPO I mRNA and TOPO II mRNA induced by Cinobufacini injection.

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Authors and Affiliations

  1. Oncology Department of the First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China

    Yu Sun, Xinxin Lu & Xiaonan Cui

  2. Academia Sinica Physiochemical Institute of Dalian, Dalian, 116011, China

    Xinmiao Liang

Authors
  1. Yu Sun
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  2. Xinxin Lu
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  3. Xinmiao Liang
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  4. Xiaonan Cui
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Corresponding author

Correspondence to Xiaonan Cui.

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Sun, Y., Lu, X., Liang, X. et al. The impact of Cinobufacini injection on proliferation and apoptosis of human hepatoma HepG-2 cells. Chin. -Ger. J. Clin. Oncol. 10, 27–30 (2011). https://doi.org/10.1007/s10330-011-0714-x

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  • DOI: https://doi.org/10.1007/s10330-011-0714-x

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