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Decreasing Pin1 suppressed the properties of migratory and invasive in colorectal carcinoma SW620 cells

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The Chinese-German Journal of Clinical Oncology

Abstract

Objective

The aim of our study was to investigate the effect of Pin1 on the expression of MMP-2 and MMP-9 in human colorectal carcinoma SW620 cells.

Methods

We constructed a eukaryotic expression vector of RNA interfering (shRNA) for Pin1 gene (pGenesil-1-Pin1), and then observed its expression in SW620 cells by Western blotting. The cells motility were tested by wound healing assay and Boyden chamber assay. The protein levels and activity of MMP-2 and MMP-9 were tested by Western blotting and Gelatin zymography in SW620 cells after transfected with pGenesil-1-PIN1.

Results

pGenesil-1-PIN1 was successfully constructed, which was confirmed by sequencing. Silencing the Pin1 by RNAi significantly decreased the cells motility from 96.4 ± 3.9 per field (× 10 objective) to 52.7 ± 4.4 per field (P < 0.05, Student’s t-test) for SW620 cells transfected with pGenesil-1-PIN1 (SW620/p-shRNA) in Boyden chamber assay, and reduced the MMP-2 and MMP-9 expressions and activity in SW620 cells. The protein relative levels of MMP-2 were 0.32 ± 0.04 for SW620/p-shRNA, and 0.76 ± 0.03 for SW620/p-Con; MMP-9 were 0.41 ± 0.09 for SW620/p-shRNA, and 0.94 ± 0.07 for SW620/p-Con (P < 0.05).

Conclusion

Inhibited Pin1 expression may contribute to the suppression of the invasive and metastatic capacity of colon cancer cells in vitro.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, 030001, China

    Liyuan Qin, Hua Hao, Meining Li, Dong Zhang, Jianlin Zhang & Niuliang Cheng

Authors
  1. Liyuan Qin
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  2. Hua Hao
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  3. Meining Li
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  4. Dong Zhang
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  5. Jianlin Zhang
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  6. Niuliang Cheng
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Corresponding author

Correspondence to Niuliang Cheng.

Additional information

Supported by a grant from the Science and Technology Project of Shanxi Province, China (No. 2006031087-02).

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Qin, L., Hao, H., Li, M. et al. Decreasing Pin1 suppressed the properties of migratory and invasive in colorectal carcinoma SW620 cells. Chin. -Ger. J. Clin. Oncol. 9, 216–220 (2010). https://doi.org/10.1007/s10330-010-0013-y

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  • DOI: https://doi.org/10.1007/s10330-010-0013-y

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