Abstract
Bacterial panicle blight (BPB) like symptoms in rice plants were first detected in Sungai Ache, Penang, Malaysia in December 2017. The symptoms observed were straw-coloured panicles, discoloured florets with darker basal portion of the glumes. The panicles remained upright due to partially or aborted grain filling. The causal pathogen was confirmed as B. glumae based on phenotypic characterization, molecular identification, and phylogenetic analysis of 16S rRNA and gyrB genes. Koch’s postulates were fulfilled and pathogenic variations among the isolates were observed. This study is the first report of the occurrence of BPB caused by B. glumae in Malaysia.
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References
Fory PA, Triplett L, Ballen C, Abello JF, Duitama J, Aricapa MG, Prado GA, Correa F, Hamilton J, Leach JE, Tohme J, Mosquera GM (2014) Comparative analysis of two emerging rice seed bacterial pathogens. Phytopathology 104:436–444. https://doi.org/10.1094/PHYTO-07-13-0186-R
Goto K, Ohata K (1956) New bacterial diseases of rice (brown stripe and grain rot). Ann Phytopathol Soc Jpn 21:46–47
Groth DE, Rush MC, Hollier CA (1991) Rice diseases and disorders in Louisiana. LSU Agricultural Experiment Station Reports 668
Ham JH, Melanson RA, Rush MC (2011) Burkholderia glumae: next major pathogen of rice? Mol Plant Pathol 12:329–339. https://doi.org/10.1111/j.1364-3703.2010.00676.x
Jeong Y, Kim J, Kim S, Kang Y, Nagamatsu T, Hwang I (2003) Toxoflavin produced by Burkholderia glumae causing rice grain rot is responsible for inducing bacterial wilt in many field crops. Plant Dis 87:890–895. https://doi.org/10.1094/PDIS.2003.87.8.890
Karki HS, Ham JH (2014) The roles of the shikimate pathway genes, aroA and aroB, in virulence, growth and UV tolerance of Burkholderia glumae strain 411gr-6. Mol Plant Pathol 15:940–947. https://doi.org/10.1111/mpp.12147
Karki HS, Shrestha BK, Han JW, Groth DE, Barphagha IK, Rush MC, Melanson RA, Kim BS, Ham JH (2012) Diversities in virulence, antifungal activity, pigmentation and DNA fingerprint among strains of Burkholderia glumae. PLoS ONE 7:45376
Kim J, Kim JG, Kang Y, Jang JY, Jog GJ, Lim JY, Kim S, Suga H, Nagamatsu T, Hwang I (2004) Quorum sensing and the LysR-type transcriptional activator ToxR regulate toxoflavin biosynthesis and transport in Burkholderia glumae. Mol Microbiol 54:921–934. https://doi.org/10.1111/j.1365-2958.2004.04338.x
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35:1547–1549
Lee J, Park J, Kim S, Park I, Seo YS (2016) Differential regulation of toxoflavin production and its role in the enhanced virulence of Burkholderia gladioli. Mol Plant Pathol 17:65–76
Maeda Y, Shinohara H, Kiba A, Ohnishi K, Furuya N, Kawamura Y, Ezaki T, Vandamme P, Tsushima S, Hikichi Y (2006) Phylogenetic study and multiplex PCR-based detection of Burkholderia plantarii, Burkholderia glumae and Burkholderia gladioli using gyrB and rpoD sequences. Int J Syst Evol Microbiol 56:1031–1038. https://doi.org/10.1099/ijs.0.64184-0
Mizobuchi R, Fukuoka S, Tsuiki C, Tsushima S, Sato H (2018) Evaluation of major Japanese rice cultivars for resistance to bacterial grain rot caused by Burkholderia glumae and identification of standard cultivars for resistance. Breed Sci 68:413–419. https://doi.org/10.1270/jsbbs.18018
Mulaw T, Wamishe Y, Jia Y (2018) Characterization and in plant detection of bacteria that cause bacterial panicle blight of rice. Am J Plant Sci 9:667–684. https://doi.org/10.4236/ajps.2018.94053
Nandakumar R, Shahjahan AKM, Yuan XL, Dickstein ER, Groth DE, Clark CA, Cartwright RD, Rush MC (2009) Burkholderia glumae and B. gladioli cause bacterial panicle blight in rice in the southern United States. Plant Dis 93:896–905. https://doi.org/10.1094/PDIS-93-9-0896
Sayler RJ, Cartwright RD, Yang Y (2006) Genetic characterization and real-time PCR detection of Burkholderia glumae, a newly emerging bacterial pathogen of rice in the United States. Plant Dis 90:603–610. https://doi.org/10.1094/PD-90-0603
Schaad NW, Jones JB, Chun W (2001) Laboratory guide for the identification of plant pathogenic bacteria. APS Press, St Paul
Seo YS, Lim JY, Choi BS, Kim H, Goo E, Lee B, Lim JS, Choi IY, Moon JS, Kim J, Hwang I (2011) Complete genome sequence of Burkholderia gladioli BSR3. J Bacteriol 193:3149
Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526
Ura H, Furuya N, Iiyama K, Hidaka M, Tsuchiya K, Matsuyama N (2006) Burkholderia gladioli associated with symptoms of bacterial grain rot and leaf-sheath browning of rice plants. J Gen Plant Pathol 72:98–103
Urakami T, Ito-Yoshida C, Araki H, Kijima T, Suzuki KI, Komagata K (1994) Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and description of Burkholderia vandii sp. nov. Int J Syst Evol Microbiol 44:235–245. https://doi.org/10.1099/00207713-44-2-235
Wamishe Y (2015) Bacterial panicle blight of rice in Arkansas. In: Arkansas row crops. University of Arkansas. http://www.arkansas-crops.com/2015/04/15/bacterial-panicle-arkansas/. Accessed 15 Mar 2020
Yamamoto S, Harayama S (1998) Phylogenetic relationships of Pseudomonas putida strains deduced from the nucleotide sequences of gyrB, rpoD and 16S rRNA genes. Int J Syst Evol Microbiol 48:813–819
Yuan X (2004) Identification of bacterial pathogens causing panicle blight of rice in Louisiana. Louisiana State University. https://digitalcommons.lsu.edu/gradschool_theses/2883. Accessed 21 Nov 2018
Zhou XG (2014) First report of bacterial panicle blight of rice caused by Burkholderia glumae in South Africa. Plant Dis 98:566–566. https://doi.org/10.1094/PDIS-09-13-0913-PDN
Zhou XG (2019) Sustainable strategies for managing bacterial panicle blight in rice. In: Yulin J (ed) Protecting rice grains in the post-genomic era. IntechOpen, London. https://doi.org/10.5772/intechopen.84882
Zhu B, Lou MM, Huai Y, Xie GL, Luo JY, Xu LH (2008) Isolation and identification of Burkholderia glumae from symptomless rice seeds. Rice Sci 15:145–149
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This research is a part of a rice development project financed by the Malaysian Agricultural Research and Development Institute (MARDI), Selangor (Project No. P21003004010001).
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Ramachandran, K., Vijaya, S.I., Ahmad, F.N. et al. Characterization and identification of Burkholderia glumae as the causal pathogen of bacterial panicle blight of rice (Oryza sativa L.) in Malaysian rice granaries. J Gen Plant Pathol 87, 164–169 (2021). https://doi.org/10.1007/s10327-021-00991-1
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DOI: https://doi.org/10.1007/s10327-021-00991-1