Skip to main content
SpringerLink
Log in

Colletotrichum and Diaporthe species associated with soybean stem diseases in Myanmar

  • Fungal Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

In 2017, premature abscission of leaves and dry rot with discoloration on the epidermis of the stem were observed in the field of reproductive-stage soybean in southern Shan State and the Nay Pyi Taw region in Myanmar. On the basis of morphological characteristics, two fungal genera, Colletotrichum and Diaporthe, were identified. Multilocus phylogenetic analyses based on the ITS, TUB and EF1-α genes for Diaporthe and the ITS, ACT, GAPDH, and CHS-1 genes for Colletotrichum were used to identify the species as Colletotrichum plurivorum, C. truncatum, Diaporthe endophytica and D. melonis. The pathogenicity of the collected isolates was confirmed by inoculation of soybean cultivar (Yezin-10), and all species except for C. plurivorum were virulent. The isolates of C. plurivorum were less aggressive than the other isolates. Koch’s postulate was fulfilled by reisolation of the original inoculated fungal isolates from the symptomatic tissue. In two locations in Myanmar, C. plurivorum, D. endophytica and D. melonis occurred in southern Shan State, whereas only one C. truncatum was found in the Nay Pyi Taw region. This is the first report of Colletotrichum and Diaporthe species associated with soybean stem diseases in Myanmar.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ash GJ, Stodart B, Sakuanrungsirikul S, Anschaw E, Crump N, Hailstones D, Harper JDI (2010) Genetic characterization of a novel Phomopsis sp., a putative biocontrol agent for Carthamus lanatus. Mycologia 102:54–61

    Article  CAS  PubMed  Google Scholar 

  • Bailey JA, Jeger MJ (eds) (1992) Colletotrichum: biology, pathology and control. CABI, Wallingford

    Google Scholar 

  • Barbieri MCG, Ciampi-Guillardi M, Moraes SRG, Bonaldo SM, Rogerio F, Linhares RR, Massola NS (2017) First report of Colletotrichum cliviae causing anthracnose on soybean in Brazil. Plant Dis 101:1677

    Article  Google Scholar 

  • CAB International (2001) Colletotrichum truncatum [distribution map]. In: Distribution maps of plant diseases 1st edn, map no. 835. https://www.cabi.org/ISC/abstract/20066500835. CABI, Wallingford, UK.

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556

    Article  CAS  Google Scholar 

  • Chen LS, Chu C, Liu CD, Chen RS, Tsay JG (2006) PCR-based detection and differentiation of anthracnose pathogens, Colletotrichum gloeosporioides and C. truncatum, from vegetable soybean in Taiwan. J Phytopathol 154:654–662

    Article  CAS  Google Scholar 

  • CSO (2017) 2017 Myanmar statistical yearbook. Central Statistical Organization, Ministry of National Planning and Economics Development, Nay Pyi Taw

    Google Scholar 

  • Damm U, Sato T, Alizadeh A, Groenewald JZ, Crous PW (2019) The Colletotrichum dracaenophilum, C. magnum and C. orchidacearum species complexes. Stud Mycol 92:1–46

    Article  CAS  PubMed  Google Scholar 

  • Damm U, Woudenberg JHC, Cannon PF, Crous PW (2009) Colletotrichum species with curved conidia from herbaceous hosts. Fungal Divers 39:45–87

    Google Scholar 

  • Dissanayake AJ, Phillips AJL, Hyde KD, Yan JY, Li XH (2017) The current status of species in Diaporthe. Mycosphere 8:1106–1156

    Article  Google Scholar 

  • Fu M, Crous PW, Bai Q, Zhang PF, Xaing J, Guo YS, Zhao FF, Yang MM, Hong N, Xu WX, Wang GP (2018) Colletotrichum species associated with anthracnose of Pyrus spp. in China. Persoonia 42:1–35

    Article  PubMed  PubMed Central  Google Scholar 

  • Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rust. Mol Ecol 2:113–118

    Article  CAS  PubMed  Google Scholar 

  • Glass LN, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gomes RR, Glienke C, Videira SIR, Lombard L, Groenewald JZ, Crous PW (2013) Diaporthe: a genus of endophytic, saprobic and plant pathogenic fungi. Persoonia 31:1–41

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120

    Article  CAS  PubMed  Google Scholar 

  • Kmetz KT, Schmitthenner AF, Ellette CW (1978) Soybean seed decay: prevalence of infection and symptom expression caused by Phomopsis sp., Diaporthe phaseolorum var. sojae, and D. phaseolorum var. caulivora. Phytopathology 68:836–840

    Article  Google Scholar 

  • Kull LS, Vuong TD, Powers KS, Eskridge KM, Steadman JR, Hartman GL (2007) Evaluation of resistance screening methods for Sclerotinia stem rot of soybean and dry bean. Plant Dis 87:1471–1476

    Article  Google Scholar 

  • Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lanfear R, Calcott B, Ho SYW, Guindon S (2012) Partitionfinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Mol Biol Evol 29:1695–1701

    Article  CAS  PubMed  Google Scholar 

  • Li S, Bradley CA, Hartman GL, Pedersen WL (2001) First report of Phomopsis longicolla from velvetleaf causing stem lesions on inoculated soybean and velvetleaf plants. Plant Dis 85:1031

    Article  CAS  PubMed  Google Scholar 

  • Li S, Hartman GL, Boykin DL (2010) Aggressiveness of Phomopsis longicolla and other Phomopsis spp. on soybean. Plant Dis 94:1035–1040

    Article  PubMed  Google Scholar 

  • Liu F, Tang G, Zheng X, Li Y, Sun X, Qi X, Zhou Y, Xu J, Chen H, Chang X, Zhang S, Gong G (2016) Molecular and phenotypic characterization of Colletotrichum species associated with anthracnose disease in peppers from Sichuan Province, China. Sci Rep 6:1–17

    Article  CAS  Google Scholar 

  • Manandhar JB, Hartman GL, Sinclair JB (1986) Colletotrichum destructivum, the anamorph of Glomerella glycines. Phytopathology 76:282–285

    Article  Google Scholar 

  • Mostert L, Crous PW, Kang J-C, Phillips AJL (2001) Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization. Mycologia 93:146–167

    Article  Google Scholar 

  • Nilsson RH, Kristiansson E, Ryberg M, Hallenberg N (2008) Intraspecific ITS variability in the kingdom Fungi as expressed in the international sequence databases and its implications for molecular species identification. Evol Bioinform 4:193–201

    Article  Google Scholar 

  • Rehner SA, Uecker FA (1994) Nuclear ribosomal internal transcribed spacer phylogeny and host diversity in the coelomycete Phomopsis. Can J Bot 72:1666–1674

    Article  CAS  Google Scholar 

  • Riccioni L, Conca G, Hartman GL (1998) First report of Colletotrichum coccodes on soybean in the United States. Plant Dis 82:959

    Article  CAS  PubMed  Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P et al (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542

    Article  PubMed  PubMed Central  Google Scholar 

  • Sakthivel K, Manigundan K, Sneha S, Patel A, Charishma K, Neelam S, Gautam RK, Kumar A (2018) First report of Colletotrichum plurivorum from the Andaman and Nicobar Islands causing anthracnose in chilli (Capsicum annuum). New Dis Rep 38:26

    Article  Google Scholar 

  • Santos JM, Vrandečić K, Ćosić J, Duvnjak T, Phillip AJL (2011) Resolving the Diaporthe species occurring on soybean in Croatia. Persoonia 27:9–19

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Consortium FB (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci 109:6241–6246

    Article  PubMed  PubMed Central  Google Scholar 

  • Shan Z, Li S, Liu Y, Yang Z, Yang C, Sha A, Chen H, Chen S, Zhou XA (2013) First report of Phomopsis seed decay of soybean caused by Phomopsis longicolla in South China. Plant Dis 96:1693

    Article  Google Scholar 

  • Shishido M, Yoshida N, Usami T, Shinozaki T, Kobayashi M, Takeuchi T (2006) Black root rot of cucurbits caused by Phomopsis sclerotioides in Japan and phylogenetic grouping of the pathogen. J Gen Plant Pathol 72:220–227

    Article  CAS  Google Scholar 

  • Singh R, Singh SB, Singh PN (2001) Effect of environmental conditions on development of anthracnose of soybean. Ann Plant Protein Sci 9:146–147

    Google Scholar 

  • Sun YC, Damm U, Huang CJ (2019) Colletotrichum plurivorum, the causal agent of anthracnose fruit rot of papaya in Taiwan. Plant Dis 11:1040

    Article  Google Scholar 

  • TeKrony D, Egli D, Stuckey R (1983) Relationship between weather and soybean seed infection by Phomopsis sp. Phytopathology 73:914–918

    Article  Google Scholar 

  • Templeton MD, Rikkerink EHA, Solon SL, Crowhurst RN (1992) Cloning and molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase-encoding gene and cDNA from the plant pathogenic fungus Glomerella cingulata. Gene 122:225–230

    Article  CAS  PubMed  Google Scholar 

  • Thaung MM (2008) Biodiversity survey of coelomycetes in Burma. Australas Mycol 27:74–110

    Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Twizeyimana M, Hill CB, Pawlowski M, Paul C, Hartman GL (2012) A cut-stem inoculation technique to evaluate soybean for resistance to Macrophomina phaseolina. Plant Dis 96:1210–1215

    Article  CAS  PubMed  Google Scholar 

  • Udayanga D, Castlebury LA, Rossman AY, Chukeatirote E, Hyde KD (2015) The Diaporthe sojae species complex: phylogenetic re-assessment of pathogens associated with soybean, cucurbits and other field crops. Fungal Biol 119:383–407

    Article  PubMed  Google Scholar 

  • Udayanga D, Castlebury LA, Rossman AY, Hyde KD (2014) Species limits in Diaporthe: molecular re-assessment of D. citri, D. cytosporellaD. foeniculina and D. rudis. Persoonia 32:83–101

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • USDA (2018) World agricultural production: crop production tables. Production, Supply and Distribution, Office of Global Analysis, Foreign Agricultural Service/USDA, Washington.

  • Weir BS, Johnston PR, Damm U (2012) The Colletotrichum gloeosporioides species complex. Stud Mycol 73:115–180

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and amplification. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Wrather JA, Anderson TR, Arsyad DM, Gai J, Ploper LD, Porta Puglia A, Ram HH, Yorinori JT (1997) Soybean disease loss estimates for the top 10 soybean producing countries in 1994. Plant Dis 81:107–110

    Article  CAS  PubMed  Google Scholar 

  • Wrather JA, Shannon G, Balardin R, Carregal L, Escobar R, Gupta GK, Ma Z, Morel W, Ploper D, Tenuta A (2010) Effect of diseases on soybean yield in the top eight producing countries in 2006. Plant Health Prog. https://doi.org/10.1094/PHP-2010-0125-01-RS

    Article  Google Scholar 

  • Yang H-C, Haudenshield JS, Hartman GL (2014) Colletotrichum incanum sp. nov., a curved-conidial species causing soybean anthracnose in USA. Mycologia 106:32–42

    Article  CAS  PubMed  Google Scholar 

  • Zhang AW, Riccioni L, Pedersen WL, Kollipara KP, Hartman GL (1998) Molecular identification and phylogenetic grouping of Diaporthe phaseolorum and Phomopsis longicolla isolates from soybean. Phytopathology 88:1306–1314

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was supported by the Japanese Government (Monbukagakusho: MEXT) scholarship programme and a Grand-in-Aid for Scientific Research (no. 18K05652) from the Japan Society for the Promotion of Science. We thank Mr. Thet Tun Aung, Programme officer, and Mr. Aye Kyaw, Soybean/oil Seed Programme Officer, Value Chains and Rural Development, Taunggyi Field Office, Winrock International, Myanmar for their support during field sample collection.

Author information

Authors and Affiliations

  1. Graduate School of Integrated Sciences for Global Society, Kyushu University, Fukuoka, Japan

    Myo Zaw

  2. Yezin Agricultural University, Nay Pyi Taw, Myanmar

    Seint San Aye

  3. Institute of Tropical Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0359, Japan

    Masaru Matsumoto

Authors
  1. Myo Zaw
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seint San Aye
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masaru Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masaru Matsumoto.

Ethics declarations

Conflict of interest

The authors have no conflicts of interest to declare.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zaw, M., Aye, S.S. & Matsumoto, M. Colletotrichum and Diaporthe species associated with soybean stem diseases in Myanmar. J Gen Plant Pathol 86, 114–123 (2020). https://doi.org/10.1007/s10327-019-00902-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-019-00902-5

Keywords

Search

Navigation