Skip to main content
SpringerLink
Log in

Dwarfing caused by viral pathogens and leaf malformations in ‘Shine Muscat’ grapevine

  • Viral and Viroid Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

‘Shine Muscat’ grapevines occasionally develop leaf malformations with chlorosis, vein clearing, and dwarfing. Grapevine fabavirus, grapevine geminivirus A, hop stunt viroid, and grapevine yellow speckle viroid 1 were detected in all six mother vines examined using next-generation sequencing and reverse transcription–polymerase chain reaction analyses, but 5 of 15 mother vine progenitors had no detectable viral pathogens. At 24 °C, leaf malformations were severe, and even clones of viral pathogen-negative vines had leaf malformations; the causes remain unclear. Infection with viral pathogens via graft inoculation from the mother vine caused severe dwarfing and severe leaf malformations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  • Al Rwahnih M, Alabi OJ, Westrick NM, Golino D, Rowhani A (2016) Near-complete genome sequence of grapevine fabavirus, a novel putative member of the genus Fabavirus. Genome Announc 4:e00703-16

    Article  Google Scholar 

  • Al Rwahnih M, Alabi OJ, Westrick NM, Golino D, Rowhani A (2017) Description of a novel monopartite geminivirus and its defective subviral genome in grapevine. Phytopathology 107:240–251

    Article  Google Scholar 

  • Fan XD, Zhang ZP, Ren F, Hu GJ, Li ZN, Dong YF (2017) First report of grapevine fabavirus in grapevines in China. Plant Dis 101:847

    Article  Google Scholar 

  • Ito T, Nakaune R (2018) Grapevine fabavirus and grapevine geminivirus A in Japan. In: Fiore N, Carrasco AZ (eds) Proceedings of the 19th Congress of ICVG, Universidad de Chile, Santiago, pp 74–75

  • Jo Y, Song MK, Choi H, Park JS, Lee JW, Cho WK (2017) First report of grapevine fabavirus in diverse Vitis species in Korea. Plant Dis 101:1829

    Google Scholar 

  • Jo Y, Choi H, Song MK, Park JS, Lee JW, Cho WK (2018) First report of grapevine geminivirus A in diverse Vitis species in Korea. Plant Dis 102:255

    Article  Google Scholar 

  • Nakaune R, Nakano M (2006) Efficient methods for sample processing and cDNA synthesis by RT-PCR for the detection of grapevine viruses and viroids. J Virol Methods 134:244–249

    Article  CAS  Google Scholar 

  • Sano T, Mimura R, Ohshima K (2001) Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid supports a grapevine origin for hop stunt disease. Virus Genes 22:53–59

    Article  CAS  Google Scholar 

  • Shimizu S, Ito T, Miyoshi T, Tachibana Y, Ito T (2011) A broad spectrum, one-step RT-PCR to detect Satsuma dwarf virus variants using universal primers targeting both segmented RNAs 1 and 2. J Gen Plant Pathol 77:326–330

    Article  Google Scholar 

  • Sugiura H, Ito T, Matsuzaki R, Nishimura R, Yamasaki A, Yakushiji H (2018) Temperature range to cause leaf malformations on ‘Shine Muscat’ grape (in Japanese). Hort Res (Japan) 17(suppl 2):159

    Google Scholar 

  • Yamada M, Yamane H, Sato A, Hirakawa N, Iwanami H, Yoshinaga K, Ozawa T, Mitani N, Shiraishi M, Yoshioka M, Nakajima I, Nakano M, Nakaune R (2008) New grape cultivar ‘Shine Muscat’ (in Japanese). Bull Natl Inst Fruit Tree Sci 7:21–38

    Google Scholar 

  • Yamada M, Yamane H, Sato A (2017) Development and extention [sic] of a new grape cultivar ‘Shine Muscat’ (in Japanese). Hort Res (Japan) 16:229–237

    Article  Google Scholar 

  • Zhang Z, Qi S, Tang N, Zhang X, Chen S, Zhu P, Ma L, Cheng J, Xu Y, Lu M, Wang H, Ding S-W, Li S, Wu Q (2014) Discovery of replicating circular RNAs by RNA-seq and computational algorithms. PLoS Pathog 10:e1004553

    Article  Google Scholar 

Download references

Acknowledgements

We are grateful to Drs. N. Mase and Y. Sato for heat therapy and shoot-tip culture and to Dr. R. Nakaune for useful advice. This study was funded by NARO.

Author information

Authors and Affiliations

  1. Grape and Persimmon Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), Akitsu, Higashihiroshima, Hiroshima, 739-2494, Japan

    Yuya Chiaki, Takao Ito, Akihiko Sato, Hiroyoshi Sugiura & Ryotaro Nishimura

Authors
  1. Yuya Chiaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takao Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Akihiko Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroyoshi Sugiura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ryotaro Nishimura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takao Ito.

Ethics declarations

Conflict of interest

All the authors belong to NARO. The authors have full control of all primary data and agree to allow the journal to review the data if requested. The authors declare that they have no conflict of interest.

Research involving human participants and animal rights

The experiments complied with the current laws of Japan. This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

The nucleotide sequence data reported here are available in the DDBJ/EMBL/GenBank databases under accession numbers LC424098, LC457511, and LC457512.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chiaki, Y., Ito, T., Sato, A. et al. Dwarfing caused by viral pathogens and leaf malformations in ‘Shine Muscat’ grapevine. J Gen Plant Pathol 86, 34–38 (2020). https://doi.org/10.1007/s10327-019-00888-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-019-00888-0

Keywords

Search

Navigation