Abstract
Ralstonia solanacearum, the devastating causal agent of potato bacterial wilt, is a soil-borne bacterium that can survive in the soil for a long time. The development of sensitive on-field detection methods for this pathogen is highly desirable due to its widespread host range and distribution. A novel nanobiosensor was thus developed to detect unamplified genomic DNA of R. solanacearum in farm soil. Gold nanoparticles functionalized with single-stranded oligonucleotides served as a probe to detect R. solanacearum genomic DNA. The advantages of this strategy include rapidity, facile usage and being a visual colorimetric method.
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Acknowledgements
This work was supported by Research Council of Tarbiat Modares University, Tehran, Iran.
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Khaledian, S., Nikkhah, M., Shams-bakhsh, M. et al. A sensitive biosensor based on gold nanoparticles to detect Ralstonia solanacearum in soil. J Gen Plant Pathol 83, 231–239 (2017). https://doi.org/10.1007/s10327-017-0721-z
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DOI: https://doi.org/10.1007/s10327-017-0721-z