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Wheat yellow mosaic virus resistance in wheat cultivar Madsen acts in roots but not in leaves

  • Viral and Viroid Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

Wheat yellow mosaic virus (WYMV), a member of Bymovirus, is a plant pathogenic virus transmitted by the soil-borne plasmodiophorid Polymyxa graminis. The in planta titer of the virus must be quantified to elucidate the dynamics of WYMV infection and the mechanism(s) underlying host resistance. Here, we describe a robust and sensitive TaqMan-based real time PCR assay based on the sequence of the WYMV coat protein gene. WYMV was undetectable in either the leaves or roots of plants of WYMV-resistant cultivar Madsen grown in infected soil. However, when Madsen leaves were mechanically inoculated with the virus, viral multiplication was not inhibited, showing that the cultivar’s resistance was ineffective in the leaf. The virus was able to move from the virus-inoculated leaf to the stem but not into the roots of Madsen, indicating that Madsen’s resistance was effective only in the roots.

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Acknowledgments

The authors acknowledge financial support by the Ministry of Agriculture, Forestry, and Fisheries of Japan (Genomics for Agricultural Innovation grant TRS1003) to T. S. and T. K. and by the Japan Society for the Promotion of Science (Research Fellowship Grant P11515) to C. L. and T. K. We thank Ning Wang and Shunzong Ning for helpful discussions, Harumi Koyama for technical assistance and Fei Dai for data analysis.

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Authors and Affiliations

  1. National Institute of Agrobiological Sciences (NIAS), Tsukuba, Ibaraki, 305-8602, Japan

    Cheng Liu, Kohei Mishina, Chao Li, Shun Sakuma, Guoxiong Chen, Mohammad Pourkheirandish & Takao Komatsuda

  2. Chuo Agricultural Experiment Station, Hokkaido Research Organization, Naganuma, Hokkaido, 069-1395, Japan

    Takako Suzuki

  3. Julius Kühn-Institute (JKI), 06484, Quedlinburg, Germany

    Antje Habekuss & Angelika Ziegler

  4. Shandong Academy of Agricultural Sciences, Crop Research Institute, 250100, Jinan, China

    Cheng Liu

  5. Institute of Crop Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, 305-8602, Japan

    Kohei Mishina & Takao Komatsuda

Authors
  1. Cheng Liu
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  2. Takako Suzuki
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  3. Kohei Mishina
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  4. Antje Habekuss
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  5. Angelika Ziegler
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  6. Chao Li
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  7. Shun Sakuma
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  8. Guoxiong Chen
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  9. Mohammad Pourkheirandish
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  10. Takao Komatsuda
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Corresponding author

Correspondence to Takao Komatsuda.

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Experiments complied with the current laws of the country in which they were performed.

There is no conflict of interest. The authors have full control of all primary data and agree to allow the journal to review their data if requested.

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Liu, C., Suzuki, T., Mishina, K. et al. Wheat yellow mosaic virus resistance in wheat cultivar Madsen acts in roots but not in leaves. J Gen Plant Pathol 82, 261–267 (2016). https://doi.org/10.1007/s10327-016-0674-7

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  • DOI: https://doi.org/10.1007/s10327-016-0674-7

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