Abstract
A new bacterial disease was observed on three Curcuma species, C. longa (turmeric), C. aromatica (wild turmeric) and C. zedoaria (zedoary) in Okinawa Prefecture, Japan in January 2014. At harvest, leaves suddenly wilted, curled and yellowed, and the whole plant finally died. The wilted plants had rotted rhizomes with internal discoloration. Creamy-whitish bacteria were isolated from the rhizomes of each Curcuma species. These bacteria were identified as Ralstonia solanacearum race 4, biovar 4 and phylotype I based on bacteriological characteristics, pathogenicity tests and DNA-based analyses. The names bacterial wilt of turmeric, wild turmeric and zedoary are proposed for the diseases.
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Acknowledgments
The authors thank Mr. F. Kawamura (Okinawa Prefectural Nanbu Agriculture Development Center, Haebaru, Okinawa, Japan) and Mr. K. Yogi (Okinawa Prefectural Hokubu Agriculture, Forestry and Fisheries Promotion Center, Nago, Okinawa, Japan) for assistance in sample collection.
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10327_2015_596_MOESM1_ESM.docx
Supplementary Fig. 1 Detection and discrimination of DNA types of Ralstonia solanacearum race 4 strains by PCR. A mixture of two primer sets (AKIF-AKIR and 21F-21R), used to identify DNA types (I and II) within race 4 strains, respectively (Horita et al. 2004; Horita and Tsuchiya 2012), were used for the PCR. PCR products were separated electrophoretically in a 1.5 % agarose gel. Lanes: 1, MAFF 107632 (tomato); 2, MAFF 301525 (tomato); 3, MAFF 107639 (ginger); 4, MAFF 107694 (siam tulip); 5–7, CA-1−3 (wild turmeric); 8–10, CL-1−3 (turmeric); 11–13, CZ-1−3 (zedoary); m, DNA molecular size marker (100-bp DNA ladder, Toyobo, Osaka, Japan). Arrow indicates the 165-bp band that was specifically amplified from race 4 strains belonging to DNA type I. (DOCX 446 kb)
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Ajitomi, A., Inoue, Y., Horita, M. et al. Bacterial wilt of three Curcuma species, C. longa (turmeric), C. aromatica (wild turmeric) and C. zedoaria (zedoary) caused by Ralstonia solanacearum in Japan. J Gen Plant Pathol 81, 315–319 (2015). https://doi.org/10.1007/s10327-015-0596-9
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DOI: https://doi.org/10.1007/s10327-015-0596-9