Abstract
The occurrence of high-molecular-weight double-stranded RNA (dsRNA) is a hallmark of RNA virus infection in plants and fungi; thus, dsRNA profiling is an attractive tool for preliminary diagnosis or characterization of novel RNA viruses. Here, we report a fast, inexpensive, and easy method to purify viral dsRNAs using a micro-spin column method based on a dsRNA isolation protocol using cellulose powder. The dsRNAs can be purified in 1 h and does not require a particular type or source of dsRNA. This method will be useful to rapidly screen tissues for viral genomic and subgenomic dsRNAs.
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Acknowledgments
The authors thank Prof. Suzuki N (Okayama University), Prof. Teraoka T and Prof. Arie T (Tokyo University of Agriculture and Technology) and Prof. Kodama M (Tottori University) for kindly providing total nucleic acid extracts or fungal isolates. The authors also thank Prof. Valverde R A (Louisiana State University) for carefully reading and correcting the manuscript. This research was supported by a Grant from the New Energy and Industrial Technology Development Organization (No. 08C46503c).
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Okada, R., Kiyota, E., Moriyama, H. et al. A simple and rapid method to purify viral dsRNA from plant and fungal tissue. J Gen Plant Pathol 81, 103–107 (2015). https://doi.org/10.1007/s10327-014-0575-6
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DOI: https://doi.org/10.1007/s10327-014-0575-6