Abstract
In 2009, chlorotic mottle and necrosis were observed on chrysanthemums (cv. Jimba) in Aomori Prefecture, Japan. A virus was isolated from the chrysanthemum plants by serial local-lesion transfer. The symptoms exhibited by the test plants, the particle morphology, the features of the protein and the potential for transmission by thrips were similar to those for Impatiens necrotic spot virus (INSV). The partial nucleotide sequences of the nucleocapsid protein gene and the 3′-untranslated sequence of the S RNA shared 99% identity with that of an INSV isolate. This report is the first of INSV infection of chrysanthemums in Japan.
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Acknowledgments
The authors thank Dr. T. Sano for critically reading this manuscript and Dr. T. Sakurai for supplying the thrips vector.
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The nucleotide sequence data reported are available in the DDBJ/ENBL/GenBank databases under the accession number AB621923.
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Kondo, T., Yamashita, K. & Sugiyama, S. First report of Impatiens necrotic spot virus infecting chrysanthemum (Chrysanthemum morifolium) in Japan. J Gen Plant Pathol 77, 263–265 (2011). https://doi.org/10.1007/s10327-011-0317-y
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DOI: https://doi.org/10.1007/s10327-011-0317-y