Skip to main content
SpringerLink
Log in

Characterization of Puccinia psidii isolates in Hawaii using microsatellite DNA markers

  • Fungal Diseases
  • Published:
Journal of General Plant Pathology Aims and scope Submit manuscript

Abstract

We studied genetic variation in Puccinia psidii, a newly introduced rust fungus in Hawaii, using microsatellite markers. All 36 Hawaiian P. psidii isolates analyzed had the same genotype at 11 microsatellite loci, while three genotypes were identified among four isolates from Florida and 11 genotypes among 18 isolates from Brazil. One of the isolates from Florida had 95% similarity to the Hawaiian isolates. We conclude that the genetically homogeneous P. psidii isolates in Hawaii might have derived from the same strain originally introduced into the Islands and are closely related to one of the strains found in Florida.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Coutinho TA, Wingfield MJ, Alfenas AC, Crous PW (1998) Eucalyptus rust: a disease with potential for serious international implications. Plant Dis 82:819–825

    Article  Google Scholar 

  • Glen M, Alfenas AC, Zauza EAV, Wingfield MJ, Mohammed C (2007) Puccinia psidii: a threat to the Australian environment and economy—a review. Australas Plant Pathol 36:1–16

    Article  Google Scholar 

  • Kawanishi T, Uematsu S, Kakishima M, Kagiwada S, Hamamoto H, Horie H, Namba S (2009) First report of rust disease on ohia and the causal fungus, Puccinia psidii, in Japan. J Gen Plant Pathol 75:428–431

    Article  Google Scholar 

  • Killgore EM, Heu RA (2007) Ohia rust. Puccinia psidii Winter. New Pest Advisory No. 05-04. Hawaii Department of Agriculture, Hawaii (http://hawaii.gov/hdoa/pi/ppc/npa-1/npa05-04-ohiarust.pdf)

  • Marlatt RB, Kimbrough JW (1979) Puccinia psidii on Pimenta dioica in south Florida. Plant Dis Rep 63:510–512

    Google Scholar 

  • Nelson RJ, Baraoidan MR, Vera Cruz CM, Yap IV, Leach JE, Mew TW, Leung H (1994) Relationship between phylogeny and pathotype for the bacterial blight pathogen of rice. Appl Environ Microbiol 60:3275–3283

    PubMed  CAS  Google Scholar 

  • Rayachhetry MB, Elliott ML, Van TK (1997) Natural epiphytotic of the rust Puccinia psidii on Melaleuca quinquenervia in Florida. Plant Dis 81:831

    Article  Google Scholar 

  • Rayachhetry MB, Van TK, Center TD, Elliott ML (2001) Host range of Puccinia psidii, a potential biological control agent of Melaleuca quinquenervia in Florida. Biol Control 22:38–45

    Article  Google Scholar 

  • Simpson JA, Thomas K, Grgurinovic CA (2006) Uredinales species pathogenic on species of Myrtaceae. Australas Plant Pathol 35:549–562

    Article  Google Scholar 

  • Tommerup IC, Alfenas AC, Old KM (2003) Guava rust in Brazil—a threat to Eucalyptus and other Myrtaceae. N Z J For Sci 33:420–428

    Google Scholar 

  • Uchida J, Zhong S, Killgore E (2006) First report of a rust disease on Ohia caused by Puccinia psidii in Hawaii. Plant Dis 90:524

    Article  Google Scholar 

  • Zhong S, Yang B, Alfenas AC (2008) Development of microsatellite markers for the guava rust fungus Puccinia psidii. Mol Ecol Res 8:348–350

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank Robert Anderson, Robert Hauff, Anne Marie LaRosa, Eloise Killgore, Janice Uchida, Min Rayachhetry, Les Szabo, and Acelino Alfenas for providing spores of Puccinia psidii or their DNA samples for this study.

Author information

Authors and Affiliations

  1. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    Shaobin Zhong & Krishna D. Puri

  2. Department of Microbiology, University of Hawaii at Manoa, Honolulu, HI, 88105, USA

    Baojun Yang

Authors
  1. Shaobin Zhong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Baojun Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Krishna D. Puri
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shaobin Zhong.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhong, S., Yang, B. & Puri, K.D. Characterization of Puccinia psidii isolates in Hawaii using microsatellite DNA markers. J Gen Plant Pathol 77, 178–181 (2011). https://doi.org/10.1007/s10327-011-0303-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10327-011-0303-4

Keywords

Search

Navigation