Abstract
Flagellin in Pseudomonas syringae is a potent elicitor of defense responses including hypersensitive cell death in dicot plants. The oligopeptides flg22 consisting of 22 conserved amino acids near the N-terminus of flagellins is reported to induce plant defense responses. Because glycosylation of the central domain of flagellin affects its elicitor activity, we investigated whether any peptide sequence in addition to flg22 is required for flagellin-induced hypersensitive reaction. A study of recombinant flagellin polypeptides indicated that the N-terminal domain including the conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana.
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Abbreviations
- HR:
-
Hypersensitive reaction
- PAMP:
-
Pathogen-associated molecular pattern
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Acknowledgments
We thank the Leaf Tobacco Research Laboratory, Japan Tobacco Inc. and the Salk Institute for providing P. syringae pv. tabaci 6605 and T-DNA tagged Arabidopsis lines for fls2 mutants, respectively. This work was supported in part by Grants-in-Aid for Scientific Research (B) (No. 18380035) and (S) (No. 15108001) from the Ministry of Education, Culture, Sports, Science and Technology of Japan, and the Okayama University COE program “Establishment of Plant Health Science”.
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Naito, K., Ishiga, Y., Toyoda, K. et al. N-terminal domain including conserved flg22 is required for flagellin-induced hypersensitive cell death in Arabidopsis thaliana . J Gen Plant Pathol 73, 281–285 (2007). https://doi.org/10.1007/s10327-007-0017-9
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DOI: https://doi.org/10.1007/s10327-007-0017-9