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Effects of thiophanate-methyl and azoxystrobin on the composition of Cercospora kikuchii populations with thiophanate-methyl-resistant strains

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Abstract

Azoxystrobin was recently registered in Japan for the control of purple seed stain of soybean caused by Cercospora kikuchii, because the pathogen has developed resistance to thiophanate-methyl. To investigate the effects of these fungicides on the frequency of C. kikuchii strains resistant to thiophanate-methyl and on the genotype structure of the population, we sowed purple-stained seeds, approximately 40% of which were infected with resistant strains, as inocula with asymptomatic seeds and applied thiophanate-methyl and azoxystrobin during the reproductive growth of soybeans. The isolation frequency of resistant strains increased more than 99% by thiophanate-methyl but was not significantly increased by azoxystrobin. In amplified fragment length polymorphism (AFLP) DNA fingerprinting, genotypic diversity was significantly decreased by thiophanate-methyl but was not affected by azoxystrobin. In addition, the similarity of the AFLP genotype structure was increased by thiophanate-methyl but not by azoxystrobin. These results suggest that thiophanate-methyl selectively inhibited the proliferation of sensitive strains, which resulted in a small number of genotypes, most of which were resistant strains. Azoxystrobin was found to nonselectively inhibit proliferation of the pathogen, which retained a large number of genotypes including thiophanate-methyl-sensitive or thiophanate-methyl-resistant strains or both.

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Authors and Affiliations

  1. National Agricultural Research Center, 3-1-1 Kannondai, Tsukuba, 305-8666, Japan

    Iori Imazaki, Hiromi Iizumi & Kouji Ishikawa

  2. Crop Research Center, Niigata Agricultural Research Institute, Nagaoka, Japan

    Masashi Sasahara

  3. Miyagi Prefecture Furukawa Agricultural Experiment Station, Furukawa, Japan

    Nobuko Yasuda

  4. National Agricultural Research Center for Tohoku Region, Daisen, Japan

    Shinzo Koizumi

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  1. Iori Imazaki
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  2. Hiromi Iizumi
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  3. Kouji Ishikawa
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  4. Masashi Sasahara
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  5. Nobuko Yasuda
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  6. Shinzo Koizumi
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Correspondence to Iori Imazaki.

Additional information

The nucleotide sequence data for the cytochrome b gene are available in the DDBJ/EMBL/GenBank databases under accession number AB231863.

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Imazaki, I., Iizumi, H., Ishikawa, K. et al. Effects of thiophanate-methyl and azoxystrobin on the composition of Cercospora kikuchii populations with thiophanate-methyl-resistant strains. J Gen Plant Pathol 72, 292–300 (2006). https://doi.org/10.1007/s10327-006-0285-9

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