Abstract
To identify possible sites of viral attenuation, the complete nucleotide sequences of two isolates of Zucchini yellow mosaic virus (ZYMV) were determined; a severe isolate Z5-1 and an attenuated isolate from Z5-1 (designated ZYMV-2002). The viral genome of both isolates consisted of 9593 nucleotides in size and contained an open reading frame encoding a single polyprotein of 3080 amino acids. Comparison of the nucleotide sequences for Z5-1 and ZYMV-2002 revealed 14 nucleotide mutations, resulting in seven amino acid substitutions with four in the HC-Pro region, two in the CI region, and one in the NIb region. These results provide a genetic basis for future manipulation of the ZYMV reverse genetics system.
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The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB188115 and AB188116
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Wang, WQ., Natsuaki, T., Kosaka, Y. et al. Comparison of the nucleotide and amino acid sequences of parental and attenuated isolates of Zucchini yellow mosaic virus. J Gen Plant Pathol 72, 52–56 (2006). https://doi.org/10.1007/s10327-005-0248-6
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DOI: https://doi.org/10.1007/s10327-005-0248-6