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Effects of excising and washing treatments on the root respiration rates of Japanese cedar (Cryptomeria japonica) seedlings

  • Short Communication
  • Published:
Journal of Forest Research

Abstract

Tree root respiration is an important component of the carbon balance in forest ecosystems; however, it is not clear whether root preparation treatments (such as excising and washing) affect root respiration measurements. Here, we aimed to compare the respiration rates of roots subjected to different treatments (i.e., washing with water vs. brushing without water, and excising vs. not excising) for 17-month-old seedlings of Cryptomeria japonica. Immediately after sampling an entire root system, the root respiration rate was measured on a mass basis using a closed static chamber system equipped with an infrared gas analyzer. We found that the respiration rates for roots that were excised 10–20 times were significantly higher than those for roots that were not excised. There was no significant difference in the root respiration rates between washing and brushing treatments. Our results indicate that large numbers of excisions (>10 times) could lead to bias in the measured changes in specific root respiration rates, and imply that differences between washing and brushing treatments do not affect the specific root respiration rate. We conclude that potential variation in recorded root respiration rates could be minimized by standardizing the root preparation technique, which should involve rapidly removing all loose soil and limiting the extent of root excision.

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References

  • Amthor JS (2000) The McCree–de Wit–Penning de Vries–Thornley respiration paradigms: 30 years later. Ann Bot 86:1–20

    Article  CAS  Google Scholar 

  • Atkin OK, Edwards EJ, Loveys BR (2000) Response of root respiration to changes in temperature and its relevance to global warming. New Phytol 147:141–154

    Article  CAS  Google Scholar 

  • Bouma TJ, Yanai RD, Elkin AD, Hartmond U, Flores-Alva DE, Eissenstat DM (2001) Estimating age-dependent costs and benefits of roots with contrasting life span: comparing apples and oranges. New Phytol 150:685–695

    Article  Google Scholar 

  • Burton AJ, Pregitzer KS, Ruess RW, Hendrik RL, Allen MF (2002) Root respiration in North American forests: effects of nitrogen concentration and temperature across biomes. Oecologia 131:559–568

    Article  Google Scholar 

  • Forest Soil Division (1976) Classification of forest soil in Japan, 1975. Bull Gov For Exp Stn 280:1–28 (in Japanese with English summary)

  • Gough CM, Seiler JR (2004) Belowground carbon dynamics in loblolly pine (Pinus taeda) immediately following diammonium phosphate fertilization. Tree Physiol 24:845–851

    Article  PubMed  CAS  Google Scholar 

  • Lipp CC, Andersen CP (2003) Role of carbohydrate supply in white and brown root respiration of ponderosa pine. New Phytol 160:523–531

    Article  Google Scholar 

  • Makita N, Hirano Y, Dannoura M, Kominami Y, Mizoguchi T, Ishii H, Kanazawa Y (2009) Fine root morphological traits determine variation in root respiration of Quercus serrata. Tree Physiol 29:579–585

    Article  PubMed  CAS  Google Scholar 

  • Makita N, Kosugi Y, Dannoura M, Takanashi S, Niiyama K, Kassim AR, Abdul Rahim N (2012) Patterns of root respiration rates and morphological traits in 13 tree species in a tropical forest. Tree Physiol 32:303–312

    Article  PubMed  Google Scholar 

  • Marsden C, Nouvellon Y, Epron D (2008) Relating coarse root respiration to root diameter in clonal Eucalyptus stands in the Republic of the Congo. Tree Physiol 28:1245–1254

    Google Scholar 

  • Marshall JD, Perry DA (1987) Basal and maintenance respiration of mycorrhizal and nonmycorrhizal root systems of conifers. Can J For Res 17:872–877

    Article  Google Scholar 

  • Pregitzer KS, Laskowski MJ, Burton AJ, Lessard VC, Zak DR (1998) Variation in sugar maple root respiration with root diameter and soil depth. Tree Physiol 18:665–670

    Article  PubMed  Google Scholar 

  • Rakonczay Z, Seiler JR, Samuelson LJ (1997) A method for the in situ measurement of fine root gas exchange of forest trees. Environ Exp Bot 37:107–113

    Article  CAS  Google Scholar 

  • Ryan MG, Hubbard RM, Pongracic S, Raison RJ, McMurtrie RE (1996) Foliage, fine-root, woody-tissue and stand respiration in Pinus radiata in relation to nitrogen status. Tree Physiol 16:333–343

    Article  PubMed  Google Scholar 

  • Teskey RO, McGuire MA (2005) CO2 transported in xylem sap affects CO2 efflux from Liquidambar styraciflua and Platanus occidentalis stems, and contributes to observed wound respiration phenomena. Trees 19:357–362

    Article  Google Scholar 

Download references

Acknowledgments

The authors thank Mr. Toshifumi Kimura and members of the laboratory at University of Hyogo, who provided useful comments on earlier drafts of the manuscript. This research was funded by a Grant-in-Aid for Japan Society for the Promotion of Science Fellows (DC1 22-2100).

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Authors and Affiliations

  1. Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Naoki Makita

  2. School of Human Science and Environment, University of Hyogo, Himeji, 670-0092, Japan

    Ryoko Yaku, Mizue Ohashi, Keisuke Fukuda & Hidetoshi Ikeno

  3. Graduate School of Environmental Studies, Nagoya University, Nagoya, 464-8601, Japan

    Yasuhiro Hirano

  4. Kansai Research Center, Forestry and Forest Products Research Institute, Kyoto, 612-0855, Japan

    Yasuhiro Hirano

Authors
  1. Naoki Makita
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  2. Ryoko Yaku
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  3. Mizue Ohashi
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  4. Keisuke Fukuda
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  5. Hidetoshi Ikeno
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  6. Yasuhiro Hirano
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Corresponding author

Correspondence to Naoki Makita.

Additional information

N. Makita and R. Yaku contributed equally to this work.

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Makita, N., Yaku, R., Ohashi, M. et al. Effects of excising and washing treatments on the root respiration rates of Japanese cedar (Cryptomeria japonica) seedlings. J For Res 18, 379–383 (2013). https://doi.org/10.1007/s10310-012-0355-0

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  • DOI: https://doi.org/10.1007/s10310-012-0355-0

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