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Isolation of endophytic streptomycetes from above- and belowground organs of Quercus serrata

  • Original Article
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Journal of Forest Research

Abstract

The occurrence and localization of endophytic actinomycetes within diverse organs of host plants provide ecological information that can be used to evaluate the significance of their spatial habitats. The aim of this study was to isolate and characterize endophytic actinomycetes in different organs of Quercus serrata. For this purpose, actinomycete isolates were obtained from surface-sterilized tissues of both symptomless seedlings and aged trees of Q. serrata and rhizosphere soil of the sampled seedlings. Thirty-five isolates with the ability to sporulate, including 4 from leaves of the aged trees, 10, 6, and 15 from leaves, stems, and roots of the seedlings, respectively, and 8 soil-derived isolates, were selected and characterized. The 16S rDNA nucleotide sequence analyses revealed that all of them belonged to the genus Streptomyces. According to a neighbor-joining phylogenetic tree constructed based on the results, the isolates of plant origin were divided into three major clades with high bootstrap values of 98 or 99 %, whereas eight soil-derived isolates were located at different positions from those of the endophyte isolates. Moreover, two larger clades were formed, one of which contained isolates derived only from aboveground parts, while the other contained isolates from all of the organs. These results suggest that the endophytic streptomycetes in Q. serrata may differ in their habitat positions (i.e., either above- or belowground parts).

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Acknowledgments

We are grateful to the late Dr. K. Nakanishi of Mie University for his valuable suggestions. We are also grateful to Ms. T. Chikada (Life Science Research Center, Center for Molecular Biology and Genetics, Mie University) for sequencing the samples, and to Dr. H. Shimada (Mie Prefectural Research Institute) and the members of the Laboratory of Forest Pathology and Mycology, Mie University, for their field assistance. This research was partly supported by a Grant-in-Aid for Exploratory Research from JSPS to SI (22658048).

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  1. M. Shimizu

    Present address: Laboratory of Plant Pathology, Faculty of Applied Biological Sciences, Gifu University, Yanagido, Gifu, 501-1193, Japan

Authors and Affiliations

  1. Laboratory of Forest Pathology and Mycology, Graduate School of Bioresources, Mie University, Tsu, Mie, 514-8507, Japan

    W. Thongsandee, Yosuke Matsuda & S. Ito

  2. Laboratory of Crop Production and Ecology, Graduate School of Bioresources, Mie University, Tsu, Mie, 514-8507, Japan

    M. Shimizu & H. Ehara

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  1. W. Thongsandee
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Correspondence to Yosuke Matsuda.

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Thongsandee, W., Matsuda, Y., Shimizu, M. et al. Isolation of endophytic streptomycetes from above- and belowground organs of Quercus serrata . J For Res 18, 179–189 (2013). https://doi.org/10.1007/s10310-012-0337-2

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  • DOI: https://doi.org/10.1007/s10310-012-0337-2

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