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RETRACTED ARTICLE: Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels

  • Fermentation, Cell Culture and Bioengineering - Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

This article was retracted on 08 February 2020

This article has been updated

Abstract

The dissolved oxygen (DO) level of a culture of Corynebacterium glutamicum (C. glutamicum) in a bioreactor has a significant impact on the cellular redox potential and the distribution of energy and metabolites. In this study, to gain a deeper understanding of the effects of DO on the metabolism of C. glutamicum, we sought to systematically explore the influence of different DO concentrations on genetic regulation and metabolism through transcriptomic analysis. The results revealed that after 20 h of fermentation, oxygen limitation enhanced the glucose metabolism, pyruvate metabolism and carbon overflow, and restricted NAD+ availability. A high oxygen supply enhanced the TCA cycle and reduced glyoxylate metabolism. Several key genes involved in response of C. glutamicum to different oxygen concentrations were examined, which provided suggestions for target site modifications in developing optimized oxygen supply strategies. These data provided new insights into the relationship between oxygen supply and metabolism of C. glutamicum.

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Change history

  • 08 February 2020

    The authors have retracted this article.

  • 08 February 2020

    The authors have retracted this article.

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Acknowledgements

This study was funded by the National Basic Research Program of China (973 Program) (Grant Number 2013CB733602), the Fundamental Research Funds for the Central Universities (Grant Number JUSRP51401A), the National Natural Science Foundation of China (Grant Number 31570034), and the Natural Science Foundation of Jiangsu Province (Grant Number BK20150148).

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    Authors and Affiliations

    1. National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China

      Xiuxia Liu, Sun Yang, Fen Wang, Xiaofeng Dai, Yankun Yang & Zhonghu Bai

    2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

      Xiuxia Liu, Sun Yang, Fen Wang, Xiaofeng Dai, Yankun Yang & Zhonghu Bai

    3. The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China

      Xiuxia Liu, Sun Yang, Fen Wang, Xiaofeng Dai, Yankun Yang & Zhonghu Bai

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    1. Xiuxia Liu
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    3. Fen Wang
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    4. Xiaofeng Dai
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    Correspondence to Yankun Yang or Zhonghu Bai.

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    This article does not contain any studies with human participants or animals performed by any of the authors.

    Additional information

    X. Liu and S. Yang are contributed equally to this work.

    The authors have retracted this article [1] because a significant portion of the study has been previously published in Chinese [2]. This article is therefore redundant. All authors agree to this retraction.

    [1] Liu, X., Yang, S., Wang, F. et al. J Ind Microbiol Biotechnol (2017) 44: 181. https://doi.org/10.1007/s10295-016-1854-3

    [2] Yang YK, Wang F, Sun Y. Effect of different dissolved oxygen concentrations on metabolism in Corynebacterium glutamicum. Microbiology China. (2016) ;43:11. 10.13344/j.microbiol.china.150975.

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    Liu, X., Yang, S., Wang, F. et al. RETRACTED ARTICLE: Comparative analysis of the Corynebacterium glutamicum transcriptome in response to changes in dissolved oxygen levels. J Ind Microbiol Biotechnol 44, 181–195 (2017). https://doi.org/10.1007/s10295-016-1854-3

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    • DOI: https://doi.org/10.1007/s10295-016-1854-3

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