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Optimization of growth medium for Sporosarcina pasteurii in bio-based cement pastes to mitigate delay in hydration kinetics

  • Natural Products
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Microbial-induced calcium carbonate precipitation has been identified as a novel method to improve durability and remediate cracks in concrete. One way to introduce microorganisms to concrete is by replacing the mixing water with a bacterial culture in nutrient medium. In the literature, yeast extract often has been used as a carbon source for this application; however, severe retardation of hydration kinetics has been observed when yeast extract is added to cement. This study investigates the suitability of alternative carbon sources to replace yeast extract for microbial-induced calcium carbonate precipitation in cement-based materials. A combination of meat extract and sodium acetate was identified as a suitable replacement in growth medium for Sporosarcina pasteurii; this alternative growth medium reduced retardation by 75 % (as compared to yeast extract) without compromising bacterial growth, urea hydrolysis, cell zeta potential, and ability to promote calcium carbonate formation.

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  1. Exchange rate: 1 USD = 0.92 €.

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Acknowledgments

The authors gratefully acknowledge Brewster Dairy, Inc. for donating the lactose mother liquor used in this study. Stephanie Chu is acknowledged for her help with the calorimetry experiments.

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Authors and Affiliations

  1. Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin, 301 East Dean Keeton Street, Stop C1700, Austin, TX, 78712, USA

    Sarah L. Williams, Mary Jo Kirisits & Raissa Douglas Ferron

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  1. Sarah L. Williams
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  2. Mary Jo Kirisits
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  3. Raissa Douglas Ferron
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Correspondence to Raissa Douglas Ferron.

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Williams, S.L., Kirisits, M.J. & Ferron, R.D. Optimization of growth medium for Sporosarcina pasteurii in bio-based cement pastes to mitigate delay in hydration kinetics. J Ind Microbiol Biotechnol 43, 567–575 (2016). https://doi.org/10.1007/s10295-015-1726-2

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  • DOI: https://doi.org/10.1007/s10295-015-1726-2

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