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Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural

  • Bioenergy/Biofuels/Biochemicals
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Furfural is a toxic by-product formulated from pretreatment processes of lignocellulosic biomass. In order to utilize the lignocellulosic biomass on isobutanol production, inhibitory effect of the furfural on isobutanol production was investigated and combinatorial application of two oxidoreductases, FucO and YqhD, was suggested as an alternative strategy. Furfural decreased cell growth and isobutanol production when only YqhD or FucO was employed as an isobutyraldehyde oxidoreductase. However, combinatorial overexpression of FucO and YqhD could overcome the inhibitory effect of furfural giving higher isobutanol production by 110 % compared with overexpression of YqhD. The combinatorial oxidoreductases increased furfural detoxification rate 2.1-fold and also accelerated glucose consumption 1.4-fold. When it compares to another known system increasing furfural tolerance, membrane-bound transhydrogenase (pntAB), the combinatorial aldehyde oxidoreductases were better on cell growth and production. Thus, to control oxidoreductases is important to produce isobutanol using furfural-containing biomass and the combinatorial overexpression of FucO and YqhD can be an alternative strategy.

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Acknowledgments

The authors thank to Prof. Oh, Min-Kyu for kind gift of E. coli strain, DSM01. The study was partially supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015M1A5A1037196) and Advanced Production Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea (1201349190011). This work was also supported by the R&D Program of MOTIE/KEIT (10048350, 10049674) and the Energy Efficiency and Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea Government Ministry of Trade, Industry and Energy (20133030000300).

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Authors and Affiliations

  1. Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea

    Hyung-Min Seo, Jong-Min Jeon, Ju Hee Lee, Hun-Suk Song, Han-Byul Joo & Yung-Hun Yang

  2. Food Ingredients Center, Foods R&D, CheilJedang, Guro-dong, Guro-gu, Seoul, 152-051, Republic of Korea

    Sung-Hee Park

  3. Department of Environmental Engineering, Ajou University, 206, World Cup-ro, Yeongtong-gu, Suwon, Gyeonggi-do, 443-749, Republic of Korea

    Kwon-Young Choi

  4. Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-gu, Taejon, 305-806, Republic of Korea

    Yong Hyun Kim, Jungoh Ahn & Hongweon Lee

  5. Department of Biological and Chemical Engineering, Hongik University, Sejong-ro 2639, Jochiwon, Sejong City, Republic of Korea

    Kyungmoon Park

  6. Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul, 143-701, Republic of Korea

    Yung-Hun Yang

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  1. Hyung-Min Seo
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Seo, HM., Jeon, JM., Lee, J.H. et al. Combinatorial application of two aldehyde oxidoreductases on isobutanol production in the presence of furfural. J Ind Microbiol Biotechnol 43, 37–44 (2016). https://doi.org/10.1007/s10295-015-1718-2

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