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Immobilization of Streptomyces thermotolerans 11432 on polyurethane foam to improve production of Acetylisovaleryltylosin

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

In this study, polyurethane foam (PUF) was chemically treated to immobilize Streptomyces thermotolerans 11432 for semi-continuous production of acetylisovaleryltylosin (AIV). Based on experimental results, positive cross-linked PUF (PCPUF) was selected as the most effective carrier according to immobilized cell mass. The effect of adsorption time on immobilized mass was investigated. AIV concentration (33.54 mg/l) in batch fermentations with immobilized cells was higher than with free cells (20.34 mg/l). In repeated batch fermentations with immobilized S. thermotolerans 11432 using PCPUF cubes, high AIV concentrations and conversion rates were attained, ranging from 25.56 to 34.37 mg/l and 79.93 to 86.31 %, respectively. Significantly, this method provides a feasible strategy for efficient AIV production and offers the potential for large-scale production.

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Acknowledgments

We are grateful for the financial support from the National Natural Science Foundation of China (Grant No. 31370091) and Natural Science Foundation of Tianjin (Grant No. 10JCYBJC10300).

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Authors and Affiliations

  1. Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Hongji Zhu, Weihua Wang, Jiaheng Liu, Qinggele Caiyin & Jianjun Qiao

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  1. Hongji Zhu
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Correspondence to Jianjun Qiao.

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Zhu, H., Wang, W., Liu, J. et al. Immobilization of Streptomyces thermotolerans 11432 on polyurethane foam to improve production of Acetylisovaleryltylosin. J Ind Microbiol Biotechnol 42, 105–111 (2015). https://doi.org/10.1007/s10295-014-1545-x

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