Skip to main content
SpringerLink
Log in

Novel extremely acidic lipases produced from Bacillus species using oil substrates

  • Biocatalysis
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The extremely acidophilic microorganisms Bacillus pumilus and Bacillus subtilis were isolated from soil collected from the commercial edible oil and fish oil extraction industry. Optimization of conditions for acidic lipase production from B. pumilus and B. subtilis using palm oil and fish oil, respectively, was carried out using response surface methodology. The extremely acidic lipases, thermo-tolerant acidic lipase (TAL) and acidic lipase (AL), were produced by B. pumilus and B. subtilis, respectively. The optimum conditions for B. pumilus obtaining the maximum activity (1,100 U/mL) of TAL were fermentation time, 96 h; pH, 1; temperature, 50 °C; concentration of palm oil, 50 g/L. After purification, a 7.1-fold purity of lipase with specific activity of 5,173 U/mg protein was obtained. The molecular weight of the TAL was 55 kDa. The AL from B. subtilis activity was 214 U/mL at a fermentation time of 72 h; pH, 1; temperature, 35 °C; concentration of fish oil, 30 g/L; maltose concentration, 10 g/L. After purification, an 11.4-fold purity of lipase with specific activity of 2,189 U/mg protein was obtained. The molecular weight of the extremely acidic lipase was 22 kDa. The functional groups of lipases were determined by Fourier transform-infrared (FT-IR) spectroscopy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

References

  1. Gombert AK, Pinto AL, Castilho LR, Freire DMG (1999) Lipase production by Penicillium restrictum in solid-state fermentation using babassu oil cake as substrate. Process Biochem 35:85–89

    Article  CAS  Google Scholar 

  2. Rudolph A (1995) Changes in the coastal environment caused by organic material from the fishing industry: a case study. J Sea Sci Technol CONA, No. Especial, pp 69–78

  3. Crine DG, Paloumet X, Bjornsson L, Alves MM, Mattiasson B (2007) Anaerobic digestion of lipid-rich waste—effects of lipid concentration. Renew Energy 32:965–975

    Article  Google Scholar 

  4. Brockman HL, Borgstorm B (1984) Lipases. Elsevier, Amsterdam, pp 3–4

  5. Lam SY, Yeung RCY, Yu TH, Sze KH, Wong KB (2011) A rigidifying salt-bridge favors the activity of thermophilic enzyme at high temperatures at the expense of low-temperature activity. PLoS Biol 9(3):e1001027. doi:10.1371/journal.pbio.1001027

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Ramani K, Saranya P, Chandan jain S, Sekaran G (2013) Lipase from marine strain using cooked sunflower oil waste: production optimization and application for hydrolysis and thermodynamic studies. Bioprocess Biosyst Eng 36:301–315

    Article  CAS  PubMed  Google Scholar 

  7. Lowry OH, Rosebrough NJ, Farr AL, Randal J (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275

    CAS  PubMed  Google Scholar 

  8. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  9. Box GEP, Hunter WG, Hunter JS (1978) Statistics for experiments. Wiley, New York

  10. Ramani K, Chockalingam Evvie, Sekaran G (2010) Production of a novel extracellular acidic lipase from Pseudomonas gessardii using slaughterhouse waste as a substrate. J Ind Microbiol Biotechnol 37:531–535

    Article  CAS  PubMed  Google Scholar 

  11. Anuradha B, Darah I, Rashidah Abdul R, Fatimah Azzahra AR (2012) Purification and characterization of a thermostable lipase form Geobacillus thermodenitrificans IBRL-nra. Enzyme Res. doi:10.1155/2012/987523

    Google Scholar 

  12. Nirupama Nawani N, Dosanjh NS, Kaur J (1998) A novel thermostable lipase from a thermophilic Bacillus sp.: characterization and esterification studies. Biotechnol Lett 20:997–1000

    Article  Google Scholar 

  13. Mahadik ND, Puntambekar US, Bastawde KB, Khire JM, Gokhale DV (2002) Production of acidic lipase by Aspergillus niger in solid state fermentation. Process Biochem 38:715–721

    Article  CAS  Google Scholar 

  14. Ramani K, John Kennedy L, Ramakrishnan M, Sekaran G (2010) Purification, characterization and application of acidic lipase from Pseudomonas gessardii using beef tallow as a substrate for fats and oil hydrolysis. Process Biochem 45:1683–1691

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Financial assistance from EMEP and INDEPTH (CSIR network project) is greatly appreciated.

Author information

Authors and Affiliations

  1. Environmental Technology Division, Council of Scientific and Industrial Research (CSIR)-Central Leather Research Institute (CLRI), Adyar, Chennai, 600 020, Tamil Nadu, India

    P. Saranya, H. Sukanya Kumari, M. Jothieswari, B. Prasad Rao & G. Sekaran

Authors
  1. P. Saranya
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Sukanya Kumari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Jothieswari
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. Prasad Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Sekaran
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. Sekaran.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saranya, P., Kumari, H.S., Jothieswari, M. et al. Novel extremely acidic lipases produced from Bacillus species using oil substrates. J Ind Microbiol Biotechnol 41, 9–15 (2014). https://doi.org/10.1007/s10295-013-1355-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-013-1355-6

Keywords

Search

Navigation