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Actinomycetes biosynthetic potential: how to bridge in silico and in vivo?

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Journal of Industrial Microbiology & Biotechnology

Abstract

Actinomycetes genome sequencing and bioinformatic analyses revealed a large number of “cryptic” gene clusters coding for secondary metabolism. These gene clusters have the potential to increase the chemical diversity of natural products. Indeed, reexamination of well-characterized actinomycetes strains revealed a variety of hidden treasures. Growing information about this metabolic diversity has promoted further development of strategies to discover novel biologically active compounds produced by actinomycetes. This new task for actinomycetes genetics requires the development and use of new approaches and tools. Application of synthetic biology approaches led to the development of a set of strategies and tools to satisfy these new requirements. In this review, we discuss strategies and methods to discover small molecules produced by these fascinating bacteria and also discuss a variety of genetic instruments and regulatory elements used to activate secondary metabolism cryptic genes for the overproduction of these metabolites.

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Acknowledgments

The work in the AL group was supported through funding from the ERC starting grant EXPLOGEN No. 281623 and the DFG grant (Lu1524/2-1). We thank Dr. Stephanie Brown (JIC, Norwich, UK) for help in manuscript preparation.

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  1. Helmholtz-Institute for Pharmaceutical Research Saarland, Campus, Building C2.3, Saarbrücken, 66123, Germany

    Yuriy Rebets, Elke Brötz, Bogdan Tokovenko & Andriy Luzhetskyy

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  1. Yuriy Rebets
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  2. Elke Brötz
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Correspondence to Andriy Luzhetskyy.

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Rebets, Y., Brötz, E., Tokovenko, B. et al. Actinomycetes biosynthetic potential: how to bridge in silico and in vivo?. J Ind Microbiol Biotechnol 41, 387–402 (2014). https://doi.org/10.1007/s10295-013-1352-9

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