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Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus

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Journal of Industrial Microbiology & Biotechnology

Abstract

We show that a previously annotated hypothetical protein is the transposase of a new and active IS element, ISCahy1, widespread in Caldicellulosiruptor species. Transposition generated an 11-bp direct repeat at the insertion site in Caldicellulosiruptor hydrothermalis, suggesting a cut-and-paste mechanism. The discovery of an active insertion sequence in Caldicellulosiruptor species led to a survey of potential IS elements in the genome sequences of eight Caldicellulosiruptor species that identified several new elements, including one novel to this genus.

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Acknowledgments

We thank Lee Lynd, Dan Olson, and Adam Guss for sharing unpublished results, Jenna Oberstaller for assistance in genome sequence analysis, and Minseok Cha and Jennifer Copeland for helpful discussions during the course of the work. This work was supported by The BioEnergy Science Center supported by a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. JF was supported in part by a predoctoral Graduate Training In Genetics grant (NIH 5T32GM007103-30) to the Genetics Department of the University of Georgia.

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Authors and Affiliations

  1. Department of Genetics, University of Georgia, Athens, GA, 30602, USA

    Daehwan Chung, Joel Farkas & Janet Westpheling

  2. The BioEnergy Science Center, Department of Energy, Oak Ridge National Laboratory, Oak Ridge, TN, USA

    Daehwan Chung, Joel Farkas & Janet Westpheling

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  1. Daehwan Chung
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  2. Joel Farkas
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  3. Janet Westpheling
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Correspondence to Janet Westpheling.

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Chung, D., Farkas, J. & Westpheling, J. Detection of a novel active transposable element in Caldicellulosiruptor hydrothermalis and a new search for elements in this genus. J Ind Microbiol Biotechnol 40, 517–521 (2013). https://doi.org/10.1007/s10295-013-1244-z

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