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Succinic acid fermentation in a stationary-basket bioreactor with a packed bed of immobilized Actinobacillus succinogenes: 1. Influence of internal diffusion on substrate mass transfer and consumption rate

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

This paper is dedicated to the study on external and internal mass transfers of glucose for succinic fermentation under substrate and product inhibitions using a bioreactor with a stationary basket bed of immobilized Actinobacillus succinogenes cells. By means of the substrate mass balance for a single particle of biocatalysts, considering the Jerusalimsky kinetic model including both inhibitory effects, specific mathematical expressions have been developed for describing the profiles of the substrate concentrations and mass flows in the outer and inner regions of biocatalyst particles, as well as for estimating the influence of internal diffusion on glucose consumption rate. The results indicated that very low values of internal mass flow could be reached in the particles center. The corresponding region was considered biologically inactive, with its extent varying from 0.24% to 44% from the overall volume of each biocatalyst. By immobilization of bacterial cells and use of a basket bed, the rate of glucose consumption is reduced up to 200 times compared with the succinic fermentation system containing free cells.

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Authors and Affiliations

  1. Department of Biotechnologies, “Gr.T. Popa” University of Medicine and Pharmacy of Iasi, M. Kogalniceanu 9-13, 700454, Iasi, Romania

    Anca-Irina Galaction & Marius Turnea

  2. Department of Biochemical Engineering, “Gheorghe Asachi” Technical University of Iasi, D. Mangeron 73, 700050, Iasi, Romania

    Lenuta Kloetzer & Dan Caşcaval

  3. Satake Center for Grain Process Engineering, University of Manchester, Oxford Road M13 9PL, Manchester, UK

    Colin Webb & Anestis Vlysidis

Authors
  1. Anca-Irina Galaction
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  2. Lenuta Kloetzer
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  3. Marius Turnea
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  4. Colin Webb
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  5. Anestis Vlysidis
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  6. Dan Caşcaval
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Correspondence to Dan Caşcaval.

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Galaction, AI., Kloetzer, L., Turnea, M. et al. Succinic acid fermentation in a stationary-basket bioreactor with a packed bed of immobilized Actinobacillus succinogenes: 1. Influence of internal diffusion on substrate mass transfer and consumption rate. J Ind Microbiol Biotechnol 39, 877–888 (2012). https://doi.org/10.1007/s10295-012-1095-z

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  • DOI: https://doi.org/10.1007/s10295-012-1095-z

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