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Fermentation of xylose into ethanol by a new fungus strain Pestalotiopsis sp. XE-1

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

A new fungus, Pestalotiopsis sp. XE-1, which produced ethanol from xylose with yield of 0.47 g ethanol/g of consumed xylose was isolated. It also produced ethanol from arabinose, glucose, fructose, mannose, galactose, cellobiose, maltose, and sucrose with yields of 0.38, 0.47, 0.45, 0.46, 0.31, 0.25, 0.31, and 0.34 g ethanol/g of sugar consumed, respectively. It produced maximum ethanol from xylose at pH 6.5, 30°C under a semi-aerobic condition. Acetic acid produced in xylose fermenting process inhibited ethanol production of XE-1. The ethanol yield in the pH-uncontrolled batch fermentation was about 27% lower than that in the pH-controlled one. The ethanol tolerance of XE-1 was higher than most xylose-fermenting, ethanol-producing microbes, but lower than Saccharomyces cerevisiae and Hansenula polymorpha. XE-1 showed tolerance to high concentration of xylose, and was able to grow and produce ethanol even when it was cultivated in 97.71 g/l xylose.

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Abbreviations

GC:

Gas chromatography

HPLC:

High-performance liquid chromatography

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Authors and Affiliations

  1. College of Life Science and Technology, Guangxi University, Daxue Road, 530004, Nanning, Guangxi, China

    Zong-wen Pang, Jing-juan Liang & Ri-bo Huang

  2. State Key Laboratory for Bioenergy and Enzyme Technology, Guangxi Academy of Sciences, 98 Daling Road, 530007, Nanning, Guangxi, China

    Ri-bo Huang

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  1. Zong-wen Pang
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  2. Jing-juan Liang
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  3. Ri-bo Huang
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Correspondence to Ri-bo Huang.

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Pang, Zw., Liang, Jj. & Huang, Rb. Fermentation of xylose into ethanol by a new fungus strain Pestalotiopsis sp. XE-1. J Ind Microbiol Biotechnol 38, 927–933 (2011). https://doi.org/10.1007/s10295-010-0862-y

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