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Sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The aim of this work was to select an efficient impeller to be used in a stirred reactor for the enzymatic hydrolysis of sugar cane bagasse. All experiments utilized 100 g (dry weight)/l of steam-pretreated bagasse, which is utilized in Brazil for cattle feed. The process was studied with respect to the rheological behavior of the biomass hydrolysate and the enzymatic conversion of the bagasse polysaccharides. These parameters were applied to model the power required for an impeller to operate at pilot scale (100 l) using empirical correlations according to Nagata [16]. Hydrolysis experiments were carried out using a blend of cellulases, β-glucosidase, and xylanases produced in our laboratory by Trichoderma reesei RUT C30 and Aspergillus awamori. Hydrolyses were performed with an enzyme load of 10 FPU/g (dry weight) of bagasse over 36 h with periodic sampling for the measurement of viscosity and the concentration of glucose and reducing sugars. The mixture presented pseudoplastic behavior. This rheological model allowed for a performance comparison to be made between flat-blade disk (Rushton turbine) and pitched-blade (45°) impellers. The simulation showed that the pitched blade consumed tenfold less energy than the flat-blade disk turbine. The resulting sugar syrups contained 22 g/l of glucose, which corresponded to 45% cellulose conversion.

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Abbreviations

du/dy :

Shear rate

N :

Rotation (r.p.m)

D :

Impeller diameter

H :

Height helpful

T :

Tank diameter

w :

Thickness pitched blade

P :

Power

η :

Apparent viscosity

ρ :

Fluid density

Θ:

Angle pitched blade

g :

Gravitation constant

N p :

Power number

N Re :

Reynolds number

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Acknowledgments

This work was supported by research and scholarship grants from the National Council for Scientific and Technological Development (CNPq) of the Brazilian Ministry of Science and Technology and by the Research and Projects Financing Agency (FINEP). Usina Vale do Rosário is gratefully acknowledged for supplying the steam-pretreated bagasse.

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Authors and Affiliations

  1. Chemistry Institute, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos 149, Bloco A, Cidade Universitária, Rio de Janeiro, RJ, CEP 21941-909, Brazil

    Leonardo Tupi Caldas Pereira, Lucas Tupi Caldas Pereira, Ricardo Sposina Sobral Teixeira & Elba Pinto da Silva Bon

  2. School of Chemical Engineering, Federal University of Rio de Janeiro (UFRJ), Avenida Athos da Silveira Ramos 149, Bloco E, Cidade Universitária, Rio de Janeiro, RJ, CEP 21941-909, Brazil

    Suely Pereira Freitas

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  1. Leonardo Tupi Caldas Pereira
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  2. Lucas Tupi Caldas Pereira
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  3. Ricardo Sposina Sobral Teixeira
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  4. Elba Pinto da Silva Bon
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Correspondence to Elba Pinto da Silva Bon.

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This article is based on a presentation at the 32nd Symposium on Biotechnology for Fuels and Chemicals.

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Pereira, L.T.C., Pereira, L.T.C., Sposina Sobral Teixeira, R. et al. Sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection. J Ind Microbiol Biotechnol 38, 901–907 (2011). https://doi.org/10.1007/s10295-010-0857-8

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  • DOI: https://doi.org/10.1007/s10295-010-0857-8

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