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Efficient chemical and enzymatic saccharification of the lignocellulosic residue from Agave tequilana bagasse to produce ethanol by Pichia caribbica

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Journal of Industrial Microbiology & Biotechnology

Abstract

Bagasse of Agave tequilana (BAT) is the residual lignocellulosic waste that remains from tequila production. In this study we characterized the chemical composition of BAT, which was further saccharified and fermented to produce ethanol. BAT was constituted by cellulose (42%), hemicellulose (20%), lignin (15%), and other (23%). Saccharification of BAT was carried out at 147°C with 2% sulfuric acid for 15 min, yielding 25.8 g/l of fermentable sugars, corresponding to 36.1% of saccharificable material (cellulose and hemicellulose contents, w/w). The remaining lignocellulosic material was further hydrolyzed by commercial enzymes, ~8.2% of BAT load was incubated for 72 h at 40°C rendering 41 g/l of fermentable sugars corresponding to 73.6% of the saccharificable material (w/w). Mathematic surface response analysis of the acid and enzymatic BAT hydrolysis was used for process optimization. The results showed a satisfactory correlation (R 2 = 0.90) between the obtained and predicted responses. The native yeast Pichia caribbica UM-5 was used to ferment sugar liquors from both acid and enzymatic hydrolysis to ethanol yielding 50 and 87%, respectively. The final optimized process generated 8.99 g ethanol/50 g of BAT, corresponding to an overall 56.75% of theoretical ethanol (w/w). Thus, BAT may be employed as a lignocellulosic raw material for bioethanol production and can contribute to BAT residue elimination from environment.

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Acknowledgments

This research was funded by CONACYT, COECYT, and C.I.C./UMSNH grants. We thank Gustavo Pacheco for comments during the manuscript preparation.

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Authors and Affiliations

  1. Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Edif. B-3, Ciudad Universitaria, CP 58030, Morelia, Michoacán, Mexico

    Jaime Saucedo-Luna, Mauro Manuel Martinez-Pacheco, Carlos Ruben Sosa-Aguirre & Jesus Campos-Garcia

  2. Facultad de Ingeniería Química, Universidad Michoacana de San Nicolás de Hidalgo, Edif. M, Ciudad Universitaria, CP 58030, Morelia, Michoacán, Mexico

    Jaime Saucedo-Luna & Agustin Jaime Castro-Montoya

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  1. Jaime Saucedo-Luna
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  2. Agustin Jaime Castro-Montoya
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  3. Mauro Manuel Martinez-Pacheco
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  4. Carlos Ruben Sosa-Aguirre
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  5. Jesus Campos-Garcia
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Correspondence to Jesus Campos-Garcia.

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Saucedo-Luna, J., Castro-Montoya, A.J., Martinez-Pacheco, M.M. et al. Efficient chemical and enzymatic saccharification of the lignocellulosic residue from Agave tequilana bagasse to produce ethanol by Pichia caribbica . J Ind Microbiol Biotechnol 38, 725–732 (2011). https://doi.org/10.1007/s10295-010-0853-z

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  • DOI: https://doi.org/10.1007/s10295-010-0853-z

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