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Exploration of geosmin synthase from Streptomyces peucetius ATCC 27952 by deletion of doxorubicin biosynthetic gene cluster

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Thorough investigation of Streptomyces peucetius ATCC 27952 genome revealed a sesquiterpene synthase, named spterp13, which encodes a putative protein of 732 amino acids with significant similarity to S. avermitilis MA-4680 (SAV2163, GeoA) and S. coelicolor A3(2) (SCO6073). The proteins encoded by SAV2163 and SCO6073 produce geosmin in the respective strains. However, the spterp13 gene seemed to be silent in S. peucetius. Deletion of the doxorubicin gene cluster from S. peucetius resulted in increased cell growth rate along with detectable production of geosmin. When we over expressed the spterp13 gene in S. peucetius DM07 under the control of an ermE* promoter, 2.4 ± 0.4-fold enhanced production of geosmin was observed.

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Abbreviations

DMAPP:

Dimethylallyl diphosphate

FPP:

Farnesyl diphosphate

GC–MS:

Gas chromatography–mass spectrometry

TLC:

Thin-layer chromatography

HPLC:

High-performance liquid chromatography

IPP:

Isopentenyl diphosphate

LB:

Luria–Bertani

ORF:

Open reading frame

PCR:

Polymerase chain reaction

PKS:

Polyketide synthase

RT-PCR:

Reverse transcription polymerase chain reaction

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Acknowledgment

This study was supported by a grant of the National R&D Program for Cancer Control, Ministry of Health & Welfare, Republic of Korea (0720260).

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Authors and Affiliations

  1. Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University, # 100, Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam, 336-708, Republic of Korea

    Bijay Singh, Tae-Jin Oh & Jae Kyung Sohng

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  1. Bijay Singh
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Correspondence to Jae Kyung Sohng.

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Singh, B., Oh, TJ. & Sohng, J.K. Exploration of geosmin synthase from Streptomyces peucetius ATCC 27952 by deletion of doxorubicin biosynthetic gene cluster. J Ind Microbiol Biotechnol 36, 1257–1265 (2009). https://doi.org/10.1007/s10295-009-0605-0

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  • DOI: https://doi.org/10.1007/s10295-009-0605-0

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