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Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

Statistical methodologies were employed to optimize submerged culture medium for the production of a novel antineoplastic compound aspergiolide A by a marine-derived fungus Aspergillus glaucus HB1-19 for the first time. Orthogonal design was preformed to determine the initial composition. Then Plackett–Burman design was applied to evaluate the influence of related nutrients, and yeast extract paste, soybean powder and sodium glutamate were confirmed as critical factors in the medium. Response surface methodology (RSM) was finally taken as an effective approach to optimize the combination of the obtained three significant factors. The predicted maximal aspergiolide A production of 62.4 mg/L appeared at the region where the concentrations of sodium glutamate, soybean powder, and yeast extract paste were 2, 1, and 1.07 g/L, respectively. Under the proposed optimized conditions, the experimental aspergiolide A production reached 71.2 mg/L. The correlation between predicted value and measured value of these experiments proved the validity of the response model. After optimization, aspergiolide A production increased 4.22 times compared to that of the original medium. Elemental analysis was finally taken into consideration, and carbon–nitrogen ratio in the medium increased from 20.1:1 to 86.6:1. This great difference was inferred as the most important reason for production enhancement by metabolic pathway analysis.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (2006AA09Z429). We are grateful to Ocean University of China for supply of the strain and the aspergiolide A standard.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Road 130, 200237, Shanghai, People’s Republic of China

    Meng-Hao Cai, Xiang-Shan Zhou, Xue-Qian Sun, Ke-Jing Tao & Yuan-Xing Zhang

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  1. Meng-Hao Cai
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  2. Xiang-Shan Zhou
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  3. Xue-Qian Sun
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  4. Ke-Jing Tao
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Correspondence to Xiang-Shan Zhou or Yuan-Xing Zhang.

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Cai, MH., Zhou, XS., Sun, XQ. et al. Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus . J Ind Microbiol Biotechnol 36, 381–389 (2009). https://doi.org/10.1007/s10295-008-0507-6

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  • DOI: https://doi.org/10.1007/s10295-008-0507-6

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