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Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The presence of melanoidins in molasses wastewater leads to water pollution both due to its dark brown color and its COD contents. In this study, a bacterial consortium isolated from waterfall sediment was tested for its decolorization. The identification of culturable bacteria by 16S rDNA based approach showed that the consortium composed of Klebsiella oxytoca, Serratia mercescens, Citrobacter sp. and unknown bacterium. In the context of academic study, prevention on the difficulties of providing effluent as well as its variations in compositions, several synthetic media prepared with respect to color and COD contents based on analysis of molasses wastewater, i.e., Viandox sauce (13.5% v/v), caramel (30% w/v), beet molasses wastewater (41.5% v/v) and sugarcane molasses wastewater (20% v/v) were used for decolorization using consortium with color removal 9.5, 1.13, 8.02 and 17.5%, respectively, within 2 days. However, Viandox sauce was retained for further study. The effect of initial pH and Viandox concentration on decolorization and growth of bacterial consortium were further determined. The highest decolorization of 18.3% was achieved at pH 4 after 2 day of incubation. Experiments on fresh or used medium and used or fresh bacterial cells, led to conclusion that the limitation of decolorization was due to nutritional deficiency. The effect of aeration on decolorization was also carried out in 2 L laboratory-scale suspended cell bioreactor. The maximum decolorization was 19.3% with aeration at KLa = 2.5836 h−1 (0.1 vvm).

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Authors and Affiliations

  1. Biotechnology Program, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok, 10330, Thailand

    Suhuttaya Jiranuntipon

  2. Department of Microbiology, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok, 10330, Thailand

    Supat Chareonpornwattana

  3. Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Phyathai Road, Patumwan, Bangkok, 10330, Thailand

    Somsak Damronglerd

  4. Laboratoire de Génie Chimique, INP-ENSIACET, 5, rue Paulin Talabot Site Basso Combo, BP 1301, Toulouse, 31106, France

    Claire Albasi & Marie-Line Delia

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  1. Suhuttaya Jiranuntipon
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  2. Supat Chareonpornwattana
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Correspondence to Supat Chareonpornwattana.

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Jiranuntipon, S., Chareonpornwattana, S., Damronglerd, S. et al. Decolorization of synthetic melanoidins-containing wastewater by a bacterial consortium. J Ind Microbiol Biotechnol 35, 1313–1321 (2008). https://doi.org/10.1007/s10295-008-0413-y

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  • DOI: https://doi.org/10.1007/s10295-008-0413-y

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