Abstract
The influence of three extracellular factors (namely, the methyl oleate dispersion in the broth, the dissolved oxygen variations, and the pH fluctuation) on the lipase production by Y. lipolytica in batch bioreactor has been investigated in different scale-down apparatus. These systems allow to reproduce the hydrodynamic phenomena encountered in large-scale equipments for the three specified factors. The effects of the extracellular factors have been observed at three distinct levels: the microbial growth, the extracellular lipase production, and the induction of the gene LIP2 encoding for the main lipase of Y. lipolytica. Among the set of environmental factors investigated, the dissolved oxygen fluctuations generated in a controlled scale-down reactor (C-SDR) have led to the more pronounced physiological effect by decreasing the LIP2 gene expression level. The other environmental factors observed in a partitioned scale-down reactor, i.e., the methyl oleate dispersion and the pH fluctuations, have led to a less severe stress traduced only by a decrease of the microbial yield and thus of the extracellular lipase specific production rate.
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Frank Delvigne is a postdoctoral researcher at the Belgian “Fond pour la recherche scientifique (FNRS-FRS)” and gratefully acknowledges the FNRS-FRS for financial supports.
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Kar T. and Delvigne F. have contributed equally to this work.
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Kar, T., Delvigne, F., Masson, M. et al. Investigation of the effect of different extracellular factors on the lipase production by Yarrowia lipolityca on the basis of a scale-down approach. J Ind Microbiol Biotechnol 35, 1053–1059 (2008). https://doi.org/10.1007/s10295-008-0382-1
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DOI: https://doi.org/10.1007/s10295-008-0382-1