Skip to main content
SpringerLink
Log in

Differentiation of Aspergillus niger by random amplification of polymorphic DNA

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The randomly amplified polymorphic DNA (RAPD) patterns of whole-cell lysates from five Aspergillus niger isolates, including one reference strain, two isolated from deep freeze, and two environmental strains from soil and plant infections, were investigated. PCR-RAPD analysis of genomic DNA was performed using eight primers (Tube-A1, Tube-A6, Tube-A17, Tube-B8, Tube-B11, Tube-B15, Tube-C5, Tube-C6). The RAPD assay discriminated between all strains. Comparison of deep freeze isolates showed identical RAPD patterns in some of the reference and environmental isolates. The data indicates that the RAPD technique is useful for fingerprinting A. niger.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig.1
Fig.2

Similar content being viewed by others

References

  1. Abdel-Fattah GM, Hammad I (2002) Production of lipase by certain soil fungi. 1: optimization of cultural conditions and genetic characterization of lipolytic strains of Aspergilli using protein patterns and random amplified polymorphic DNA (RAPD). J Biol Sci 2:639–644 (Online)

    Google Scholar 

  2. Atlas RM (1993) In: Parks LC (ed) A hand book of Microbiological media. CRC Press, London, p 279

  3. Bart-Delabesse E, Sarfati J, Debeaupuis JP, Van Leeuwen W, Van Belkum A, Bretagne S, et al. (2001) Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates. J Clin Microbiol 39:2683–2686. doi:10.1128/JCM.39.7.2683-2686.2001

    Article  PubMed  CAS  Google Scholar 

  4. Birch M, Anderson MJ, Denning DW (1995) Molecular typing of Aspergillus species. J Hosp Infect 30(Suppl.):339–351

    Article  PubMed  Google Scholar 

  5. De Hoog GS, Guarro J, Gene J, Figueras MJ (2000) Atlas of clinical fungi. Utrecht-Madrid, Centralbureau voor SCHIMMELCULTURE-Universitat Rovira I Virgili-Reus

  6. Dice LR (1945) Measures of the amount of ecologic association between species. Ecology 26:297–302. doi:10.2307/1932409

    Article  Google Scholar 

  7. Fungaro MHP, Vissotto PC, Sartori D, Vilas-Boas LA, Furlaneto MC, Taniwaki MH (2004) A molecular method for detection of Aspergillus carbonarius in coffee beans. Curr Microbiol 49:123–127. doi:10.1007/s00284-004-4273-z

    Article  PubMed  CAS  Google Scholar 

  8. Gilman JC (1957) A manual of soil fungi (Second Indian Reprint). Oxford and IBH Publishing Company, New Delhi

  9. Hesseltine CW, Ellis JJ (1973) Mucorales. In: Ainsworth GC, Sparrow FK, Sussman AS (eds) The fungi: an advanced treatise. Academic Press, New York

    Google Scholar 

  10. Lasker BA (2002) Evaluation of performance of four genotypic methods for studying the genetic epidemiology of Aspergillus fumigatus isolates. J Clin Microbiol 40:2886–2892. doi:10.1128/JCM.40.8.2886-2892.2002

    Article  PubMed  CAS  Google Scholar 

  11. Lass-Florl C, Grif K, Kontoyiannis DP (2007) Molecular typing of Aspergillus terreus isolates collected in Houston, Texas, and Innsbruck, Austria: evidence of great genetic diversity. J Clin Microbiol 45:2686–2690. doi:10.1128/JCM.00917-07

    Article  PubMed  CAS  Google Scholar 

  12. Moubasher AH (1993) Soil fungi in Qatar and other Arab countries. The Centre Scientific and Applied Research, Qatar

  13. Myoken Y, Sugata T, Fujita Y, Kyo T, Fujihara M, Kohara T, et al. (2003) Molecular epidemiology of invasive stomatitis due to Aspergillus flavus in patients with acute leukemia. J Oral Pathol Med 32:215–218. doi:10.1034/j.1600-0714.2003.00056.x

    Article  PubMed  CAS  Google Scholar 

  14. Raclavsky V, Trtkova J, Ruskova L, Buchta VBR, Vackova M, Hamal P (2006) Primer R 108 performs best in the RAPD strain typing of three Aspergillus species frequently isolated from patients. Folia Microbiol (Praha) 51:136–140

    Article  CAS  Google Scholar 

  15. Raper KB, Fennell DJ (1965) The Genus Aspergillus. Williams & Wilkins, Baltimore, p 76

  16. Raper KB, Thorn CH (1949) A manual of the Genus Penicillium. Williams & Wilkins, Baltimore, pp 709–795

  17. Rath PM, Petermeier K, Verweij PE, Ansorg R (2002) Differentiation of Aspergillus ustus strains by random amplification of polymorphic DNA. J Clin Microbiol 40:2231–2233. doi:10.1128/JCM.40.6.2231-2233.2002

    Article  PubMed  CAS  Google Scholar 

  18. Rippon JW (1982) Medical mycology: the pathogenic fungi and the pathogenic Actinomycetes. Saunders, Philadelphia

  19. Samson RA, Stolk A, Hadlok R (1976) Revision on the subsection fasciculates of genus Penicillium and some allied species. Stud Mycol 11:1–47

    Google Scholar 

  20. Soll DR (2000) The ins and outs of DNA fingerprinting the infectious fungi. Clin Microbiol Rev 13:332–370. doi:10.1128/CMR.13.2.332-370.2000

    Article  PubMed  CAS  Google Scholar 

  21. Steffan P, Vazquez JA, Boikov D, Xu C, Sobel JD, Akins RA (1997) Identification of Candida species by randomly amplified polymorphic DNA fingerprinting of colony lysates. J Clin Microbiol 35:2031–2039

    PubMed  CAS  Google Scholar 

  22. Symoens F, Bouchara JP, Heinemann S, Nolard N (2000) Molecular typing of Aspergillus terreus isolates by random amplification of polymorphic DNA. J Hosp Infect 44:273–280. doi:10.1053/jhin.1999.0707

    Article  PubMed  CAS  Google Scholar 

  23. Welsh J, McClelland M (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218. doi:10.1093/nar/18.24.7213

    Article  Google Scholar 

  24. Wiederhold NP, Lewis RE, Kontoyiannis DP (2003) Invasive aspergillosis in patients with hematologic malignancies. Pharmacotherapy 23:1592–1610. doi:10.1592/phco.23.15.1592.31965

    Article  PubMed  Google Scholar 

  25. Williams JGK, Kubelike AR, Livak KJ, Rafalsk JA, Tingery SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535. doi:10.1093/nar/18.22.6531

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botany Department, University of Riyadh, Riyadh, Saudi Arabia

    Kawther F. Abed

Authors
  1. Kawther F. Abed
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kawther F. Abed.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abed, K.F. Differentiation of Aspergillus niger by random amplification of polymorphic DNA. J Ind Microbiol Biotechnol 35, 1027–1032 (2008). https://doi.org/10.1007/s10295-008-0378-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-008-0378-x

Keywords

Search

Navigation