Abstract
Penicillium strain isolated from citrus fruit was found to produce thermostable polygalacturonases. Optimization of process parameters resulted in high levels of enzyme production after 3 days of incubation at a pH of 5.0 at 30 °C in the presence of 1% pectin. The optimum temperature for enzyme activity was 60 °C and a pH of 5.5 was found to be the optimal pH. The enzyme showed a high level of thermostability in the presence of substrate with a residual activity of 48% after 2 h of incubation at 60 °C. A thermostable nature with a high pH range for activity makes it an industrially important enzyme.
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Mathew, A., Eldo, A.N. & Molly, A.G. Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20. J Ind Microbiol Biotechnol 35, 1001–1005 (2008). https://doi.org/10.1007/s10295-008-0375-0
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DOI: https://doi.org/10.1007/s10295-008-0375-0