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Fed batch bioconversion of 2-propanol by a solvent tolerant strain of Alcaligenes faecalis entrapped in Ca-alginate gel

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

A gram-negative, rod-shaped, aerobe, capable of converting 2-propanol (isopropanol, IPA) to acetone was isolated from an oil/sump, and identified by 16 S rDNA analysis as Alcaligenes faecalis. Investigations showed this strain to be extremely solvent-tolerant and it was subsequently named ST1. In this study, A. faecalis ST1 cells were immobilized by entrapment in Ca-alginate beads (3 mm in diameter), and used in the bioconversion of high concentration IPA. The biodegradation rates and the corresponding microbial growth inside the beads were measured at four different IPA concentration ranges from 2 to 15 g l−1. The maximum cell concentration obtained was 9.59 g dry cell weight (DCW) l−1 medium which equated to 66 g DCW l−1 gel, at an initial IPA concentration of 15 g l−1 after 216 h of incubation. A maximum biodegradation rate of 0.067 g IPA g cells−1 h−1 was achieved for 5 g l−1 IPA where an increase in IPA concentration to 38 g l−1 caused reduction in bead integrity. A modified growth medium was developed which allowed repeated use of the beads for more than 42 days without any loss of integrity and continued bioconversion activity.

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Acknowledgments

MTB would like to acknowledge the UK’s Royal Academy of Engineering for provision of a Research Fellowship.

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Authors and Affiliations

  1. Chemical Engineering Laboratory, Faculty of Sciences, University of La Coruna, Campus da Zapateira, 15071, La Coruna, Spain

    Balsam T. Mohammad

  2. Biochemical Engineering Group, Chemical Engineering, School of Engineering and Physical Sciences, Heriot Watt University, Edinburgh, EH14 4AS, UK

    Mark T. Bustard

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Correspondence to Balsam T. Mohammad.

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Mohammad, B.T., Bustard, M.T. Fed batch bioconversion of 2-propanol by a solvent tolerant strain of Alcaligenes faecalis entrapped in Ca-alginate gel. J Ind Microbiol Biotechnol 35, 677–684 (2008). https://doi.org/10.1007/s10295-008-0325-x

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  • DOI: https://doi.org/10.1007/s10295-008-0325-x

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