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Culture conditions improving the production of jadomycin B

  • Original Paper
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Journal of Industrial Microbiology and Biotechnology

Abstract

The jadomycins are a unique family of benzoxazolophenanthridine antibiotics produced by Streptomyces venezuelae ISP5230 following heat or ethanol shock or phage infection. We have modified the culture conditions by altering the carbon source, buffer, inoculum size, and timing of ethanol shock, thereby reducing growing times and improving jadomycin B production. Our optimized conditions use glucose as the carbon source, MOPS as buffer, low concentrations of phosphate, a defined inoculum concentration and an immediate ethanol shock to induce jadomycin B production; results that contrast previous studies. The altered media will facilitate the isolation of related jadomycin B congeners.

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References

  1. Ayer SW, McInnes AG, Thibault P, Wang L, Doull JL, Parnell T, Vining LC (1991) Jadomycin, a novel 8H-benz[B]oxazolo[3,2-F]phenanthridine antibiotic from Streptomyces venezuelae ISP5230. Tetrahedron Lett 32:6301–6304

    Article  CAS  Google Scholar 

  2. Doull JL, Singh AK, Hoare M, Ayer SW (1994) Conditions for the production of jadomycin B by Streptomyces venezuelae ISP5230: effects of heat shock, ethanol treatment and phage infection. J Ind Microbiol 13:120–125

    Article  PubMed  CAS  Google Scholar 

  3. Doull JL, Ayer SW, Singh AK, Thibault P (1993) Production of a novel polyketide antibiotic, jadomycin B, by Streptomyces venezuelae following heat shock. J Antibiot 46:849–871

    Google Scholar 

  4. Jakeman DL, Farrell S, Young W, Doucet RJ, Timmons SC (2005) Novel jadomycins: incorporation of non-natural and natural amino acids. Bioorg Med Chem Lett 15:1447–1449

    Article  PubMed  CAS  Google Scholar 

  5. Jakeman DL, Graham CL, Reid TR (2005) Novel and expanded jadomycins incorporating non-proteogenic amino acids. Bioorg Med Chem Lett 15:5280–5283

    Article  PubMed  CAS  Google Scholar 

  6. Martin JF (2004) Phosphate control of the biosynthesis of antibiotics and other secondary metabolites is mediated by the PhoR-PhoP system: an unfinished story. J Bacteriol 186:5197–5201

    Article  PubMed  CAS  Google Scholar 

  7. Rix U, Zheng J, Remsing Rix LL, Greenwell L, Yang K, Rohr J (2004) The dynamic structure of jadomycin B and the amino acid incorporation step of its biosynthesis. J Am Chem Soc 126:4496–4497

    Article  PubMed  CAS  Google Scholar 

  8. Slater H, Crow M, Everson L, Salmond GP (2003) Phosphate availability regulates biosynthesis of two antibiotics, prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent and -independent pathways. Mol Microbiol 47:303–320

    Article  PubMed  CAS  Google Scholar 

  9. Syvitski RT, Borissow CN, Graham C, Jakeman DL (2006) Ring opening dynamics of jadomycin A and B, and dalomycin T. Org Lett 8:697–700

    Article  PubMed  CAS  Google Scholar 

  10. Wang L, White RL, Vining LC (2002) Biosynthesis of the dideoxysugar component of jadomycin B: genes in the jad cluster of Streptomyces venezuelae ISP5230 for l-digitoxose assembly and transfer to the angucycline aglycone. Microbiology 148:1091–1103

    PubMed  CAS  Google Scholar 

  11. Zheng JT, Rix U, Zhao L, Mattingly C, Adams V, Quan C, Rohr J, Yang KQ (2005) Cytotoxic activities of new jadomycin derivatives. J Antibiot 58:405–408

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

We thank the Atlantic Chapter of the Canadian Breast Cancer Foundation, the Pharmacy Endowment Fund at Dalhousie University, the Canadian Institutes for Health Research and the Canada Foundation for Innovation for financial support.

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Authors and Affiliations

  1. College of Pharmacy, Dalhousie University, 5968 College Street, Halifax, NS, Canada, B3H 3J5

    David L. Jakeman, Cathy L. Graham & Wendy Young

  2. Department of Biology, Dalhousie University, 1355 Oxford St., Halifax, NS, Canada, B3H 4J1

    Leo C. Vining

Authors
  1. David L. Jakeman
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  2. Cathy L. Graham
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  3. Wendy Young
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  4. Leo C. Vining
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Correspondence to David L. Jakeman.

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Jakeman, D.L., Graham, C.L., Young, W. et al. Culture conditions improving the production of jadomycin B. J IND MICROBIOL BIOTECHNOL 33, 767–772 (2006). https://doi.org/10.1007/s10295-006-0113-4

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  • DOI: https://doi.org/10.1007/s10295-006-0113-4

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