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Proteases production by two Vibrio species on residuals marine media

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Journal of Industrial Microbiology and Biotechnology

Abstract

A comparative study was carried out on the growth and production of alkaline proteases by two Vibrio species using different marine peptones from fish viscera residues. The bacteria tested, Vibrio anguillarum and Vibrio splendidus, are producers of high levels of proteolytic enzymes which act as factors of virulence in fish cultures, causing high mortality rates. The kinetic assays and subsequent comparison with the parameters obtained from the adjustment to various mathematical models, highlighted the potential interest of the media formulated, for their possible production on an industrial scale, particularly the production of proteases by V. anguillarum growing in rainbow trout and squid peptones.

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Acknowledgements

We wish to thank the Xunta de Galicia (PGIDIT04TAM007001CT) and UE-Life Program (BE-FAIR LIFE05 ENV/E/000267-BE-FAIR) for financial support. Thanks also to Ana Durán, Margarita Nogueira and Araceli Menduiña for technical assistance. Dr. José Antonio Vázquez Álvarez had a postdoctoral contract (CSIC-I3P-PC 2003, financed by the European Social Fund). The raw materials were kindly supplied by the following companies: Alimentos Arousa S.A. (yellowfin tuna), Clavo Congelados S.A. (squid), the fish market of the port of Vigo (swordfish) and Isidro de la Cal S.A. (trout).

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  1. Instituto de Investigacións Mariñas (CSIC), r/Eduardo Cabello, 6 Vigo, 36208, Galicia, Spain

    J. A. Vázquez, S. F. Docasal, J. Mirón, M. P. González & M. A. Murado

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  1. J. A. Vázquez
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  2. S. F. Docasal
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  3. J. Mirón
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  4. M. P. González
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Correspondence to J. A. Vázquez.

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Vázquez, J.A., Docasal, S.F., Mirón, J. et al. Proteases production by two Vibrio species on residuals marine media. J IND MICROBIOL BIOTECHNOL 33, 661–668 (2006). https://doi.org/10.1007/s10295-006-0096-1

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  • DOI: https://doi.org/10.1007/s10295-006-0096-1

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