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Characterization of product capture resin during microbial cultivations

  • Original Paper
  • Published:
Journal of Industrial Microbiology and Biotechnology

Abstract

Various bioactive small molecules produced by microbial cultivation are degraded in the culture broth or may repress the formation of additional product. The inclusion of hydrophobic adsorber resin beads to capture these products in situ and remove them from the culture broth can reduce or prevent this degradation and repression. These product capture beads are often subjected to a dynamic and stressful microenvironment for a long cultivation time, affecting their physical structure and performance. Impact and collision forces can result in the fracturing of these beads into smaller pieces, which are difficult to recover at the end of a cultivation run. Various contaminating compounds may also bind in a non-specific manner to these beads, reducing the binding capacity of the resin for the product of interest (fouling). This study characterizes resin bead binding capacity (to monitor bead fouling), and resin bead volume distributions (to monitor bead fracture) for an XAD-16 adsorber resin used to capture epothilone produced during myxobacterial cultivations. Resin fouling was found to reduce the product binding capacity of the adsorber resin by 25–50%. Additionally, the degree of resin bead fracture was found to be dependent on the cultivation length and the impeller rotation rate. Microbial cultivations and harvesting processes should be designed in such a way to minimize bead fragmentation and fouling during cultivation to maximize the amount of resin and associated product harvested at the end of a run.

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Abbreviations

d :

resin bead diameter (cm)

d i :

impeller diameter (cm)

ICS:

impeller collision severity function (g cm2/s3)

n :

impeller rotational speed (revolutions/s)

TCS:

turbulent bead collision severity function (g cm2/s3)

v :

velocity of bead sized eddies in the turbulent fluid (cm/s)

v tip :

velocity of fluid over the outer edge of the impeller blade (cm/s)

V :

volume of bioreactor (cm3)

α :

volume fraction of beads (dimensionless)

ε :

turbulent power dissipation per mass of liquid (cm2/s3)

ρ b :

density of XAD-16 adsorber resin beads (g/cm3)

ν :

kinematic viscosity of medium (cm2/s)

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Acknowledgments

The authors thank Geoffrey Nosarati for his assistance with the GC/MS analysis of the resin extract samples.

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Authors and Affiliations

  1. Department of Process Science, Kosan Biosciences Inc., 3832 Bay Center Place, Hayward, CA, 94545, USA

    Scott Frykman, Hiroko Tsuruta, Jorge Galazzo & Peter Licari

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  1. Scott Frykman
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  2. Hiroko Tsuruta
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  3. Jorge Galazzo
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  4. Peter Licari
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Correspondence to Scott Frykman.

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Frykman, S., Tsuruta, H., Galazzo, J. et al. Characterization of product capture resin during microbial cultivations. J IND MICROBIOL BIOTECHNOL 33, 445–453 (2006). https://doi.org/10.1007/s10295-006-0088-1

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  • DOI: https://doi.org/10.1007/s10295-006-0088-1

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