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Evaluation of Streptomyces sp. strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets

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Journal of Industrial Microbiology and Biotechnology

Abstract

Streptomyces sp. strain g10 exhibited strong antagonism towards Fusarium oxysporum f.sp. cubense (Foc) races 1, 2 and 4 in plate assays by producing extracellular antifungal metabolites. Treating the planting hole and roots of 4-week-old tissue-culture-derived ‘Novaria’ banana plantlets with strain g10 suspension (108 cfu/ml), significantly (P<0.05) reduced wilt severity when the plantlets were inoculated with 104 spores/ml Foc race 4. The final disease severity index for leaf symptom (LSI) and rhizome discoloration (RDI) was reduced about 47 and 53%, respectively, in strain g10-treated plantlets compared to untreated plantlets. Reduction in disease incidence was not significant (P<0.05) when plantlets were inoculated with a higher concentration (106 spores/ml) of Foc race 4. Rhizosphere population of strain g10 showed significant (P=0.05) increase of more than 2-fold at the end of the 3rd week compared to the 2nd week after soil amendment with the antagonist. Although the level dropped, the rhizosphere population at the end of the 6th week was still nearly 2-fold higher than the level detected after 2 weeks. In contrast, the root-free population declined significantly (P=0.05), nearly 4-fold after 6 weeks when compared to the level detected after 2 weeks. Neither growth-inhibiting nor growth-stimulating effects were observed in plantlets grown in strain g10-amended soil.

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References

  1. AbdulKadir AM (2001) Early evaluation of banana cultivars for Fusarium wilt tolerance. MSc Thesis. University Malaya, Kuala Lumpur

  2. Baker KF, Cook RJ (1974) Biological control of plant pathogens. Freeman, San Francisco

    Google Scholar 

  3. Bell AA, Mace ME (1981) Biochemistry and physiology of resistance. In: Mace ME et al (eds) Fungal wilt diseases of plants. Academic Press, New York, pp 431–486

    Google Scholar 

  4. Brake VM, Pegg KG, Irwin JA, Chaseling J (1995) The influence of temperature, inoculum level and race of Fusarium oxysporum f.sp. cubense on the disease reaction of banana cv. Cavendish. Aust J Agric Res 46:673–685

    Article  Google Scholar 

  5. Broadbent P, Baker KF, Waterworth Y (1971) Bacteria and actinomycetes antagonistic to fungal root pathogens in Australian soils. Aust J Biol Sci 24:925–944

    Google Scholar 

  6. Brown ME (1974) Seed and root bacterization. Annu Rev Phytopathol 12:181–197

    Article  Google Scholar 

  7. Chamberlain K, Crawford DL (1999) In vitro and in vivo antagonism of pathogenic turfgrass fungi by Streptomyces hygroscopicus strains YCED9 and WYE53. J Ind Microbiol Biotechnol 23:641–646

    Google Scholar 

  8. Chambers SC, Millington JR (1974) Studies on Fusarium species associated with a field planting of ‘pathogen-tested’ potatoes. Aust J Agric Res 25:293–297

    Article  Google Scholar 

  9. Crawford DL, Lynch JM, Whipps JM, Ousley MA (1993) Isolation and characterization of actinomycete antagonists of a fungal root pathogen. Appl Environ Microbiol 59:3899–3905

    Google Scholar 

  10. El-Shanshoury AR, Abu El-Sououd SM, Awadalla OA, El-Bandy NB (1996) Effects of Streptomyces corchorusii, Streptomyces mutabilis, pendimethalin, and metribuzin on the control of bacterial and Fusarium wilt of tomato. Can J Bot 74:1016–1022

    Google Scholar 

  11. El-Tarabily KA, Sykes ML, Kurtboke ID, Hardy GESJ, Barbosa AM, Dekker RFH (1996) Synergistic effects of a cellulase-producing Micromonospora carbonacea and an antibiotic-producing Streptomyces violascens on the suppression of Phytophthora cinnamomi root rot of Banksia grandis. Can J Bot 74:618–624

    Google Scholar 

  12. Gesheva V (2002) Rhizosphere microflora of some citrus as a source of antagonistic actinomycetes. Eur J Soil Biol 38:85–88

    Article  Google Scholar 

  13. Getha K, Vikineswary S, Goodfellow M, Ward A, Seki T (2001) Inhibitory effects of an antagonistic streptomycete on Fusarium wilt pathogen of banana. In: Murooka Y et al (eds) Proceedings of the JSPS-NRCT/DOST/LIPI/VCC joint seminar: biotechnology for sustainable utilization of biological resources in the tropics. International Center for Biotechnology, Osaka University, Osaka, Japan, pp 150–153

    Google Scholar 

  14. Getha K, Vikineswary S (2002) Antagonistic effects of Streptomyces violaceusniger strain g10 on Fusarium oxysporum f.sp. cubense race 4: indirect evidence for the role of antibiosis in the antagonistic process. J Ind Microbiol Biotechnol 28:303–310

    Article  Google Scholar 

  15. Hervas A, Landa B, Datnoff LE, Jimenez-Diaz RM (1998) Effects of commercial and indigenous microorganisms on Fusarium wilt development in chickpea. Biol Control 13:166–176

    Article  Google Scholar 

  16. Hwang SC, Ko WH (1990) Tissue culture plantlets as a source of resistance to fusarial wilt of Cavendish banana. In: Hornby D (ed) Biological control of soil-borne plant pathogens. CAB International, Wallingford, UK, pp 345–353

    Google Scholar 

  17. Hwang BK, Lim SW, Kim BS, Lee JY, Moon SS (2001) Isolation and in vivo and in vitro antifungal activity of phenylacetic acid and sodium phenylacetate from Streptomyces humidus. Appl Environ Microbiol 67:3739–3745

    Article  Google Scholar 

  18. Kortemaa H, Rita H, Haahtela K, Smolander A (1994) Root-colonization ability of antagonistic Streptomyces griseoviridis. Plant Soil 163:77–83

    Google Scholar 

  19. Kurtboke DI (2000) Australian actinomycetes: an unexhausted source for biotechnological applications. Actinomycetology 14:43–53

    Google Scholar 

  20. Lahdenpera ML (1987) The control of Fusarium wilt on carnation with a Streptomyces preparation. Acta Hortic 216:85–92

    Google Scholar 

  21. Mak C, Ho YW, Tan YP, Ibrahim R (1996) Novaria a new banana mutant induced by gamma irradiation. Infomusa 5:35–36

    Google Scholar 

  22. Marois JJ (1990) Biological control of diseases caused by Fusarium oxysporum. In: Ploetz RC (ed) Fusarium wilt of banana. APS, St. Paul, Minn.

    Google Scholar 

  23. Mehrotra RS, Caludius GR (1972) Biological control of the root rot and wilt diseases of Lens culinaris Medic. Plant Soil 36:657–664

    Google Scholar 

  24. Meredith CH (1946) Soil actinomycetes applied to banana plants in the field. Phytopathology 36:983–987

    Google Scholar 

  25. Miller HJ, Henken G, van Veen JA (1989) Variation and composition of bacterial populations in the rhizospheres of maize, wheat and grass cultivars. Can J Microbiol 35:656–660

    Google Scholar 

  26. Mohamed ZK (1982) Physiological and antagonistic activities of Streptomycetes in rhizosphere of some plants. Egypt J Phytopathol 14:121–128

    Google Scholar 

  27. Nelson PE (1981) Life cycle and epidemiology of Fusarium oxysporum. In: Mace ME et al (eds) Fungal wilt diseases of plants. Academic, New York, pp 51–80

    Google Scholar 

  28. Raguchander T, Jayashree K, Samiyappan R (1997) Management of Fusarium wilt of banana using antagonistic microorganisms. J Biol Control 11:101–105

    Google Scholar 

  29. Rangaswami G, Vidyasekaran P (1963) Antibiotic production by Streptomyces sp. in corn rhizosphere. Phytopathology 53:995–997

    Google Scholar 

  30. Reddi GS, Rao AS (1971) Antagonism of soil actinomycetes to some soil-borne plant pathogenic fungi. Indian Phytopathol 24:649–657

    Google Scholar 

  31. Rothrock CS, Gottlieb D (1984) Role of antibiosis in antagonism of Streptomyces hygroscopicus var. geldanus to Rhizoctonia solani in soil. Can J Microbiol 30:1440–1447

    Google Scholar 

  32. Ryder MH, Jones DA (1993) Biological control of crown gall. In: Hornby D (ed) Biological control of soil-borne plant pathogens. CAB International, UK, pp 45–63

    Google Scholar 

  33. Sardi P, Saracchi M, Quaroni S, Petrolini B, Borgonivi GE, Merli S (1992) Isolation of endophytic Streptomyces strains from surface-sterilized roots. Appl Environ Microbiol 58:2691–2693

    Google Scholar 

  34. Sivamani E, Gnanamanickam SS (1988) Biological control of Fusarium oxysporum f.sp. cubense in banana by inoculation with Pseudomonas fluorescens. Plant Soil 107:3–9

    Google Scholar 

  35. Slininger PJ, VanCauwenberge JE, Shea-Wilbur MA, Bothast RJ (1998) Impact of liquid culture physiology, environment and metabolites on biocontrol agent qualities. In: Boland GJ, Kuykendall LD (eds) Plant-microbe interactions and biological control. Dekker, New York, pp 329–353

    Google Scholar 

  36. Smith J, Putnam A, Nair M (1990) In vitro control of Fusarium diseases of Asparagus officinalis L. with a Streptomyces or its polyene antibiotic, faeriefungin. J Agric Food Chem 38:1729–1733

    Google Scholar 

  37. Stevenson IL (1956a) Antibiotic activity of actinomycetes in soil and their controlling effects on root-rot of wheat. J Gen Microbiol 14:440–448

    Google Scholar 

  38. Stevenson IL (1956b) Antibiotic activity of actinomycetes in soil as demonstrated by direct observation techniques. J Gen Microbiol 15:372–380

    Google Scholar 

  39. Stover RH, Waite BH (1954) Colonization of banana roots by Fusarium oxysporum f.sp. cubense and other soil fungi. Phytopathology 44:689–693

    Google Scholar 

  40. Toussoun TA, Nelson PE (1968) A pictorial guide to the identification of Fusarium species according to the taxonomic system of Snyder and Hansen. The Pennsylvania State University Press, University Park, pp 1–51

    Google Scholar 

  41. Trejo-Estrada SR, Sepulveda IR, Crawford DL (1998) In vitro and in vivo antagonism of Streptomyces violaceusniger YCED9 against fungal pathogens of turfgrass. World J Microbiol Biotechnol 14:865–872

    Article  Google Scholar 

  42. Tu CC, Chang YC, Cheng YH, Lin CH, Li JH, Yuang S (1980) Studies on biological control of Panama disease (Fusarium wilt) of banana. Tainan Dais Res Bull 14:1–12

    Google Scholar 

  43. Turhan G (1981) A new race of Streptomyces ochraceiscleroticus in the biological control of some soil-borne plant pathogens. II. In vivo studies on the possibilities of using C/2-9 against some important diseases. Z Pflanzenkr Pflanzenschutz 88:421–434

    Google Scholar 

  44. Valois D, Fayad K, Barasubiye T, Garon M, Dery C, Brzezinski R, Beaulieu C (1996) Glucanolytic actinomycetes antagonistic to Phytophthora fragariae var. rubi, the causal agent of raspberry root-rot. Appl Environ Microbiol 62:1630–1635

    CAS  Google Scholar 

  45. Weller DM (1988) Biological control of soil borne plant pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407

    Article  Google Scholar 

  46. Windels CE (1992) Fusarium. In: Singleton LL et al. (eds) Methods for research on soil borne phytopathogenic fungi. APS, St. Paul, Minn., pp 115–128

    Google Scholar 

  47. Yuan WM, Crawford DL (1995) Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Appl Environ Microbiol 61:3119–3128

    Google Scholar 

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Acknowledgements

This study was supported by IRPA grant no. 08-02-03-0144 from the Ministry of Science, Technology and the Environment, Malaysia. We gratefully thank the Japanese Society for Promotion of Science, Japan and British Council, UK for facilitating the collaborations. We also thank University Malaya for their generous support.

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Authors and Affiliations

  1. Forest Research Institute, Kepong, 52109, Kuala Lumpur, Malaysia

    K. Getha

  2. Institute of Biological Sciences, University Malaya, 50603, Kuala Lumpur, Malaysia

    S. Vikineswary

  3. Sepang Institute of Technology, 41050, Klang, Selangor Darul Ehsan, Malaysia

    W. H. Wong

  4. International Center for Biotechnology, Osaka University, 565-0871, Osaka, Japan

    T. Seki

  5. Department of Agricultural and Environmental Science, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK

    A. Ward & M. Goodfellow

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  1. K. Getha
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  2. S. Vikineswary
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  5. A. Ward
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Correspondence to S. Vikineswary.

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Getha, K., Vikineswary, S., Wong, W.H. et al. Evaluation of Streptomyces sp. strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets. J IND MICROBIOL BIOTECHNOL 32, 24–32 (2005). https://doi.org/10.1007/s10295-004-0199-5

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  • DOI: https://doi.org/10.1007/s10295-004-0199-5

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