Abstract
A thermostable glucoamylase (GA) showed optimum activity at 70°C and pH 5.0. It was highly stable at pH 7.0. The half-life of the enzyme at pH 7.0 was 13, 8, and 3 h 40 min at 60, 65, and 70°C respectively. The residual activity of the enzyme sample incubated at 5 psi (110°C) for 30 min was about 32% of the control set (incubated at 4°C), while no activity was observed at 10 and 15 psi. The thermostability of the enzyme was enhanced twofold in the presence of 0.5% (w/v) starch at 5 psi. Thin-layer chromatography indicated that this enzyme is a GA.
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JRF to RKG from Council of Scientific & Industrial Research, India is acknowledged.
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Gill, R.K., Kaur, J. A thermostable glucoamylase from a thermophilic Bacillus sp.: characterization and thermostability. J IND MICROBIOL BIOTECHNOL 31, 540–543 (2004). https://doi.org/10.1007/s10295-004-0185-y
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DOI: https://doi.org/10.1007/s10295-004-0185-y