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Optimization of Serratia marcescens lipase production for enantioselective hydrolysis of 3-phenylglycidic acid ester

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Journal of Industrial Microbiology and Biotechnology

Abstract

Lipase production and cell growth of Serratia marcescens ECU1010 were optimized in shake flasks, with lipase production being enhanced 9.5-fold (4,780 U/l) compared with the initial activity (500 U/l). Optimal carbon and nitrogen sources were Tween-80 and peptone, and the optimal ratio of Tween-80 to peptone was 1:3. The optimized cultivation conditions were 25°C and pH 6.5. Lipase activity, particularly specific activity, could be improved by decreasing the cultivation temperature from 35 to 25°C. Enzyme stability was significantly improved by simple immobilization with synthetic adsorption resin no. 8244. After five reaction cycles, enzyme activity decreased only very slightly, while enantioselectivity of the preparation remained constant, and the ee s (enantiomeric excess of the remaining substrate) achieved in all cases was higher than 97%. The resin-8244-lipase preparation can be used for efficient enantioselective hydrolysis of trans-3-(4′-methoxyphenyl)glycidic acid methyl ester [(±)-MPGM], a key intermediate in the synthesis of Diltiazem.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (grant nos. 20272013 and 203900506), the Ministry of Education, P.R. China (a program for supporting excellent young teachers) and the Science and Technology Commission of Shanghai Municipal Government (grant no. 02QMF1412).

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Authors and Affiliations

  1. Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Li Gao & Jian-He Xu

  2. Laboratory of Bioseparation Engineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Xin-Jun Li & Zuo-Zhen Liu

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  1. Li Gao
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  2. Jian-He Xu
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  3. Xin-Jun Li
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  4. Zuo-Zhen Liu
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Correspondence to Jian-He Xu.

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Gao, L., Xu, JH., Li, XJ. et al. Optimization of Serratia marcescens lipase production for enantioselective hydrolysis of 3-phenylglycidic acid ester. J IND MICROBIOL BIOTECHNOL 31, 525–530 (2004). https://doi.org/10.1007/s10295-004-0182-1

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