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Cheese whey: an alternative growth and protective medium for Rhizobium loti cells

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Journal of Industrial Microbiology and Biotechnology

Abstract

Cheese whey (CW)-based growth medium efficiently protects Rhizobium loti cells during freezing and desiccation and can maintain their growth in a manner similar to that of traditional mannitol-based medium (YEM). The cheese-whey-based medium (CW) improved viability when used to re-suspend cell pellets kept at −20 °C and −80 °C and resulted in the survival of over 90% of the cells. Moreover, bacterial pellets obtained from cells grown in CW withstand desiccation better than cells grown in YEM. Survival was over 60% after 30 days at 4 °C. No differences were observed in nodulation efficiency between YEM-grown and CW-grown cells. Fast protein liquid chromatography (FPLC) protocols are presented for total protein profile analyses of sweet and acid cheese whey.

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Acknowledgements

This work was supported by a grant from the Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET-Argentina). MJE is a professional researcher and a member of the Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC). FLP is a fellow of Fundación Consejo Regional de la Producción (COREPRO). LED and OAR are career researchers of CONICET. We are grateful to M. Rodriguez (Gándara S.A., Chascomús. Argentina), who provided the cheese whey samples and collaborated in their analysis. We also wish to thank Professor Rex Davis for his help with English.

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Authors and Affiliations

  1. Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico de Chascomús (IIB-INTECh), Universidad Nacional de General San Martín-Consejo Nacional de Investigaciones Científicas y Técnicas (UNSAM-CONICET), Chascomús, Buenos Aires, Argentina

    M. J. Estrella, F. L. Pieckenstain, M. Marina & O. A. Ruiz

  2. Cátedra de Química Analítica Instrumental, Universidad Nacional de Buenos Aires (UBA), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina

    L. E. Díaz

  3. IIB-INTECh, Casilla de Correo 164, (7130) , Chascomús, Argentina

    O. A. Ruiz

Authors
  1. M. J. Estrella
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  2. F. L. Pieckenstain
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  3. M. Marina
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  4. L. E. Díaz
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  5. O. A. Ruiz
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Corresponding author

Correspondence to O. A. Ruiz.

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In memoriam of Sylvio Cortina Vicepresident of Fundación COREPRO

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Estrella, M.J., Pieckenstain, F.L., Marina, M. et al. Cheese whey: an alternative growth and protective medium for Rhizobium loti cells. J IND MICROBIOL BIOTECHNOL 31, 122–126 (2004). https://doi.org/10.1007/s10295-004-0124-y

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  • DOI: https://doi.org/10.1007/s10295-004-0124-y

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