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Selective antimicrobial action of chitosan against spoilage yeasts in mixed culture fermentations

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Journal of Industrial Microbiology and Biotechnology

Abstract

The effect of chitosan on Saccharomyces cerevisiae (the yeast that carries out alcohol fermentation), Brettanomyces bruxellensis and Brettanomyces intermedius (contaminants of alcohol fermentations), was investigated. The effect of chitosan was tested on each yeast, as well as on mixed cultivations of S. cerevisiae + B. bruxellensis and S. cerevisiae + B. intermedius. Chitosan enhanced the lag period of both strains of Brettanomyces (80 h for B. bruxellensis and 170 h for B. intermedius with 6 and 2 g/l chitosan, respectively). The growth rate of S. cerevisiae was inversely proportional to the chitosan concentration; the former was 50% when 6 g/l polysaccharide was used. Moreover, in mixed cultivations of S. cerevisiae and Brettanomyces strains, it was found that both B. bruxellensis and B. intermedius failed to grow while growth of S. cerevisiae was not affected (using 3 and 6 g/l chitosan, respectively). An interesting collateral result was that the presence of chitosan accelerated the consumption of glucose in the mixed cultivations (60 h instead of 120 h).

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Acknowledgements

This work was supported by grant 576.01-P from COSNET. H.L. Gómez-Rivas was a graduate student supported by CONACYT.

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Authors and Affiliations

  1. Departamento de Ingeniería Química-Bioquímica, Unidad de Investigación en Alimentos (UNIDA), Instituto Tecnológico de VeracruzMiguel Angel de Quevedo 2779, Col Formando Hogar, CP 91860, Veracruz, Mexico

    Leticia Gómez-Rivas, Blanca I. Escudero-Abarca, M. Guadalupe Aguilar-Uscanga, Patricia Mendoza & Mario Ramírez

  2. Universidad Veracruzana, Area Químico-Biológica, Veracruz, Mexico

    Patricia M. Hayward-Jones

Authors
  1. Leticia Gómez-Rivas
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  2. Blanca I. Escudero-Abarca
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  3. M. Guadalupe Aguilar-Uscanga
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  4. Patricia M. Hayward-Jones
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  5. Patricia Mendoza
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  6. Mario Ramírez
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Correspondence to Blanca I. Escudero-Abarca.

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Gómez-Rivas, L., Escudero-Abarca, B.I., Aguilar-Uscanga, M.G. et al. Selective antimicrobial action of chitosan against spoilage yeasts in mixed culture fermentations. J IND MICROBIOL BIOTECHNOL 31, 16–22 (2004). https://doi.org/10.1007/s10295-004-0112-2

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  • DOI: https://doi.org/10.1007/s10295-004-0112-2

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