Skip to main content
SpringerLink
Log in

Role of the pectinolytic enzyme in the lactic acid fermentation of potato pulp by Rhizopus oryzae

  • Short Communication
  • Published:
Journal of Industrial Microbiology and Biotechnology

Abstract.

Rhizopus oryzae strain NBRC 4707 produced lactic acid and ethanol more efficiently than strain NRRL 395 in potato pulp, an agricultural by-product of the starch industry. The two strains developed comparable activities of xylanase, cellulase, α-amylase, and glucoamylase, while the polygalacturonase activity of strain NBRC 4707 was double that of strain NRRL 395. The addition of commercial pectinase enhanced the formation of metabolites, suggesting that the degradation of pectic substances determines the fermentation of potato pulp by R. oryzae. Orange and apple peel were more effective in the induction of polygalacturonase activity than potato pulp, sugarbeet pulp, or wheat bran when used as a principal carbon source for fungal growth in a solid-state culture. The fungal cells in both types of fruit peel stimulated the fermentation of potato pulp and increased the quantity of lactic acid and ethanol to higher levels than those in other agricultural by-products.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.

References

  1.  Alkorta I, Garbisu C, Llama ML, Serra JL (1998) Industrial applications of pectic enzymes: a review. Proc Biochem 33:21–28

    Article  CAS  Google Scholar 

  2.  Blandino A, Dravillas K, Cantero D, Pandiella SS, Webb C (2001) Utilisation of whole wheat flour for the production of extracellular pectinases by some fungal strains. Proc Biochem 37:497–503

    Article  Google Scholar 

  3.  Blandino A, Iqbalsyah T, Pandiella SS, Cantero D, Webb C (2002) Polygalacturonase production by Aspergillus awamori on wheat in solid-state fermentation. Appl Microbiol Biotechnol 58:164–169

    CAS  PubMed  Google Scholar 

  4.  Dartora AB, Bertolin TE, Bilibio D, Silveira MM, Costa JAV (2002) Evaluation of filamentous fungi and inducers for the production of endo-polygalacturonase by solid-state fermentation. Z Naturforsch Teil C 57:666–670

    CAS  Google Scholar 

  5.  Goldberg I, Peleg Y, Rokem JS (1991) Citric, fumaric, and malic acids. In: Goldberg I, Williams R (eds) Biotechnology and food ingredients. Van Nostrand Reinhold, New York, pp 349–374

  6.  Hang YD (1989) Direct fermentation of corn tol(+)-lactic acid by Rhizopus oryzae. Biotechnol Lett 11:299–300

    CAS  Google Scholar 

  7.  Hellín P, Ros JM, Laencina J (2001) Changes in high and low molecular weight carbohydrates during Rhizopus nigricans cultivation of lemon peel. Carbohydr Polym 45:169–174

    Article  Google Scholar 

  8.  Kashyap DR, Vohra PK, Chopra S, Tewari R (2001) Application of pectinases in the commercial sector: a review. Bioresour Technol 77:215–227

    CAS  PubMed  Google Scholar 

  9.  Lockwood LB, Ward GE (1936) The physiology of Rhizopus oryzae. J Agric Res 53:849–857

    CAS  Google Scholar 

  10.  Lorrungruang C, Elegado FB, Fujio Y (1993) Some properties of polygalacturonase from Rhizopus japonicus IFO 5318. J Fac Agric Kyushu Univ 37:297–305

    CAS  Google Scholar 

  11.  Mayer F, Hillebrandt HO (1997) Potato pulp: microbiological characterization, physical modification, and application of this agricultural waste product. Appl Microbiol Biotechnol 48:435–440

    CAS  PubMed  Google Scholar 

  12.  McKay AM (1988) A plate assay method for the detection of fungal polygalacturonase secretion. FEMS Microbiol Lett 56:355–358

    Article  CAS  Google Scholar 

  13.  Nelson NJ (1957) Colorimetric analysis of sugars. Methods Enzymol 3:85–86

    Google Scholar 

  14.  Oda Y, Ichinose Y, Yamauchi H (2002a) Utilization of Lactobacillus amylovorus as an alternative microorganism for saccharifying boiled rice. Food Sci Technol Res 8:166–168

    Google Scholar 

  15.  Oda Y, Saito K, Yamauchi H, Mori M (2002b) Lactic acid fermentation of potato pulp by the fungus Rhizopus oryzae. Curr Microbiol 45:1–4

    Article  CAS  PubMed  Google Scholar 

  16.  Oda Y, Yajima Y, Kinoshita M, Ohnishi M (2003) Differences of Rhizopus oryzae strains in organic acid synthesis and fatty acid composition. Food Microbiol 20:371–375

    Article  Google Scholar 

  17.  Saito K, Kondo K, Kojima I, Yokota A, Tomita F (2000) Purification and characterization of 2,6-β-d-fructan-6-levanbiohydrolase from Streptomyces exfoliatus F3-2. Appl Environ Microbiol 66:252–256

    CAS  PubMed  Google Scholar 

  18.  Seale DR (1986) Bacterial inoculants as silage additives. J Appl Bacteriol 61:9S–26S

    Google Scholar 

  19.  Skory CD (2000) Isolation and expression of lactate dehydrogenase genes from Rhizopus oryzae. Appl Environ Microbiol 66:2343–2348

    PubMed  Google Scholar 

  20.  Skory CD, Freer SN, Bothast RJ (1998) Production ofl-lactic acid by Rhizopus oryzae under oxygen-limiting conditions. Biotechnol Lett 20:191–194

    Article  CAS  Google Scholar 

  21.  Van Dijk C, Beekhuizen JG, Gibcens T, Boeriu C, Fischer M, Stolle-Smits T (2002) Texture of cooked potatoes (Solanum tuberosum). 2. Changes in pectin composition during storage of potatoes. J Agric Food Chem 50:5089–5097

    Article  PubMed  Google Scholar 

  22.  Woiciechowski AL, Soccol CR, Ramos LP, Pandey A (1999) Experimental design to enhance the production ofl-(+)-lactic acid from steam-exploded wood hydrolysate using Rhizopus oryzae in a mixed-acid fermentation. Proc Biochem 34:949–955

    Article  CAS  Google Scholar 

  23.  Woolford MK (1984) The silage fermentation. Dekker, New York

  24.  Yin P, Nishiya N, Kosakai Y, Yahiro K, Park YS, Okabe M (1997) Enhanced production ofl(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor. J Ferment Bioeng 84:249–253

    Article  CAS  Google Scholar 

Download references

Acknowledgement.

This work was partially supported by the Special Coordination Funds for Promoting Science and Technology (Leading Research Utilizing Potential of Regional Science and Technology) of the Japanese Ministry of Education, Culture, Sports, Science, and Technology.

Author information

Authors and Affiliations

  1. Department of Upland Agriculture, National Agricultural Research Center for the Hokkaido Region, Memuro, 082-0071, Hokkaido, Japan

    K. Saito, Y. Kawamura & Y. Oda

Authors
  1. K. Saito
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Kawamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Oda
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Y. Oda.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saito, K., Kawamura, Y. & Oda, Y. Role of the pectinolytic enzyme in the lactic acid fermentation of potato pulp by Rhizopus oryzae . J IND MICROBIOL BIOTECHNOL 30, 440–444 (2003). https://doi.org/10.1007/s10295-003-0071-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10295-003-0071-z

Keywords.

Search

Navigation