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Production of the glycopeptide antibiotic A40926 by Nonomuraea sp. ATCC 39727: influence of medium composition in batch fermentation

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Journal of Industrial Microbiology and Biotechnology

Abstract

Nonomuraea sp. ATCC 39727 is a novel actinomycete species and the producer of A40926, a glycopeptide antibiotic structurally similar to teichoplanin. In the present study, a defined minimal medium was designed for Nonomuraea fermentation. The influence of initial phosphate, glucose and ammonium concentrations on antibiotic productivity was investigated in batch fermentation and the effect of glucose limitation was studied in fed-batch fermentation. It was found that low initial concentrations of phosphate and ammonium are beneficial for A40926 production and that productivity is not enhanced during glucose limitation. Furthermore, the initiation of A40926 production was not governed by residual ammonium and phosphate concentrations, although the level of these nutrients strongly influenced A40926 production rates and final titers.

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Acknowledgements

We thank Flavia Marinelli and Srdjan Jovetic, Biosearch Italia SpA, for advice on the handling of the Nonomuraea strain and Einar Jonsbu for providing helpful comments on medium design for actinomycetes. This work was financed by the European Union via the MEGA-TOP project QLK3-1999-00650; and we acknowledge the scientific discussions with our colleagues participating in this project.

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  1. Per Bruheim

    Present address: Department of Biotechnology, Norwegian University of Science and Technology, Trondheim, Norway

Authors and Affiliations

  1. Center for Process Biotechnology, Biocentrum-DTU, Building 223, Søltofts plads, 2800, Lyngby, Denmark

    Nina Gunnarsson, Per Bruheim & Jens Nielsen

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  1. Nina Gunnarsson
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  2. Per Bruheim
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  3. Jens Nielsen
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Correspondence to Jens Nielsen.

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Gunnarsson, N., Bruheim, P. & Nielsen, J. Production of the glycopeptide antibiotic A40926 by Nonomuraea sp. ATCC 39727: influence of medium composition in batch fermentation. J IND MICROBIOL BIOTECHNOL 30, 150–156 (2003). https://doi.org/10.1007/s10295-003-0024-6

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  • DOI: https://doi.org/10.1007/s10295-003-0024-6

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