Abstract
Ten species of Aspergillus isolated from soil samples collected from different locations in the Indian Himalayan region have been studied for their growth requirements and tricalcium phosphate solubilization at different temperatures. The Aspergillus species could grow at low temperature and tolerated a wide range of pH. Phosphate solubilization by various Aspergillus species ranged between 374 μg/ml (A. candidus) to 1394 μg/ml (A. niger) at 28°C, 33 μg/ml (A. fumigatus) to 2354 μg/ml (A. niger) at 21°C, 93 μg/ml (A. fumigatus) to 1452 μg/ml (A. niger) at 14°C, and 21 μg/ml (A. wentii) to 83 μg/ml (A. niger) at 9°C. At 21 and 28°C, phosphate solubilization showed a decrease within 4 weeks of incubation, whereas at 9°C and 14°C, it continued further up to 6 weeks of incubation. In general, phosphate solubilization by different Aspergillus species was recorded at a maximum of 28°C or 21°C; biomass production was favored at 21°C or 14°C. Conversely, A. nidulans and A. sydowii exhibited maximum phosphate solubilization at 14°C and produced maximum biomass at 21°C. Data suggest that suboptimal conditions (higher or lower temperature) for fungal growth and biomass production were optimal for the production of metabolites involved in phosphate solubilization. Significant negative correlations were obtained between pH and phosphate solubilization for eight species at 28°C, for seven at 21°C, and for nine at 14°C. Extracellular phosphatase activity was exhibited only in case of A. niger, whreas intracellular phosphatase activity was detected in all species, the maximum being in A. niger. Statistically significant positive or negative correlations were obtained between phosphate solubilization and other parameters in most cases at different temperatures.
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References
Aleksieva P, Spasova D, Radoevska S (2003) Acid phosphatase distribution and localization in the fungus Humicola lutea. Z Naturforsch 58:239–243
Banik S, Dey BK (1982) Available phosphate content of an alluvial soil as influenced by inoculation of some isolated phosphate-solubilizing micro-organisms. Plant Soil 69:353–364
Barroso CB, Pereira GT, Nahas E (2006) Solubilization of CaHPO4 and AlPO4 by Aspergillus niger in culture media with different carbon and nitrogen sources. Braz J Microbiol 37:434–438
Braibant M, Content J (2001) The cell surface associated phosphatase activity of Mycobacterium bovis BCG is not regulated by environmental inorganic phosphate. FEMS Microbiol Lett 195:121–126
Cunningham JE, Kuiack C (1992) Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium billai. Appl Environ Microbiol 52:1451–1458
De Croos JNA, Bidochka MJ (2001) Cold induced proteins in cold-active isolates of the insect-pathogenic fungus Metarhizium anisopliae. Mycol Res 105:868–873
Gadd GM (1999) Fungal production of citric and oxalic acid: Importance in metal speciation, physiology and biochemical processes. Adv Microb Physiol 41:47–92
Gaur AC (1990) Phosphate solubilizing microorganisms as biofertilizer. Omega scientific publishers, New Delhi
Goenadi DH, Siswanto, Sugiarto Y (2000) Bioactivation of poorly soluble phosphate rocks with a phosphorus-solubilizing fungus. Soil Sci Soc Am J 64:927–932
Goldstein AH (1995) Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by Gram-negative bacteria. Biol Agric Hortic 12:185–193
Illmer P, Schinner F (1992) Solubilization of inorganic phosphates by microorganisms isolated from forest soils. Soil Biol Biochem 24:389–395
Illmer P, Barbato A, Schinner F (1995) Solubilization of hardly-soluble AlPO4 with P solubiling microorganisms. Soil Biol Biochem 27:265–270
Jackson ML (1967) Soil Chemical analysis. Prentice Hall of India, India
Klich MA (2002) Biogeography of Aspergillus species in soil and litter. Mycologia 94:21–27
Michael OG, Robert CE (1984) Fungal nutrition and physiology. Wiley, Canada
Omar SA (1998) The role of rock phosphate-solubilizing fungi and vesicular arbuscular-mycorrhiza (VAM) in growth of wheat plants fertilized with rock phosphate. World J Microbiol Biotechnol 14:211–218
Pandey A, Palni LMS (2007) The rhizosphere effect in the trees of the Indian Central Himalaya with special reference to altitude. Appl Ecol Environ Res 5:93–102
Pandey A, Palni LMS, Mulkalwar P, Nadeem M (2002) Effect of temperature on solubilization of tricalcium phosphate by Pseudomonas corrugata. J Sci Ind Res 61:457–460
Pandey A, Trivedi P, Kumar B, Palni LMS (2006a) Characterization of a phosphate solubilizing and antagonistic strain of Pseudomonas putida (B0) isolated from a sub-alpine location in the Indian Central Himalaya. Curr Microbiol 53(2):102–107
Pandey A, Trivedi P, Kumar B, Chaurasia B, Palni LMS (2006b) Soil microbial diversity from the Himalaya: need for documentation and conservation. National Biodiversity Authority Scientific Bulletin No. 5, Chennai, Tamil Nadu
Pandey A, Das N, Kumar B, Rinu K, Trivedi P (2008) Phosphate solubilization by Penicillium spp. isolated from soil samples of Indian Himalayan region. World J Microbiol Biotechnol 24:97–102
Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17:362–370
Ramalingam N, Prasanna BG (2006) Effect of aluminum phosphate on alkaline phosphatase activity of polyurethane foam immobilized cyanobacteria. Curr Microbiol 53:194–197
Reyes I, Bernier L, Baziramakenga R, Antoun H (2001) Solubilization of phosphate rocks and minerals by a wild-type strain and two UV-induced mutants of Penicillium rugulosum. Soil Biol Biochem 33:1741–1747
Richardson A, Hadobas PA, Hayes JE (2000) Acid phosphomonoesterase and phytase activities of wheat (Trticum aestivum L) roots and utilization of organic phosphorus substrates by seedlings grown in sterile culture. Plant Cell Environ 23:397–405
Salih HM, Yahya AI, Abdul-Rahem AM, Munam BH (1989) Availability of phosphorus in a calcarious soil treated with rock phosphate or superphosphate as affected by phosphate dissolving fungi. Plant Soil 120:181–185
Shieh TR, Wodzinski RJ, Ware JH (1969) Regulation of the formation of acid phosphatases by inorganic phosphate in Aspergillus ficcum. J Bacteriol 100:1161–1165
Sperber JI (1958) Solution of apatite by microorganisms producing organic acids. Aust J Agric Res 9:782–787
SPSS/PC (1986) SPSS/PC for the IBM PC/XT/AT. SPSS Inc., Illinois
Tabatabai MA, Bremner JM (1969) Use of p-Nitrophenol phosphate for assay of soil phosphatase activity. Soil Biol Biochem 1:301–307
Tarafdar JC, Jungk A (1987) Phosphatase activity in the rhizosphere and its relation to the depletion of soil organic phosphorus. Biol Fertil Soils 3:199–204
Tarafdar JC, Bareja M, Panwar J (2003) Efficiency of some phosphatase producing soil-fungi. Indian J Microbiol 43:27–32
Vassileva M, Vassilev N, Azcon R (1998) Rock phosphate solubilization by Aspergillus niger on olive cake based medium and its further application in a soil-plant system. World J Microbiol Biotechnol 14:281–284
Velazquez E, Rodriguez-Barrueco C (2007) First international meeting on microbial phosphate solubilization. In: Developments in Plant and Soil Sciences, vol 102. Springer, Salamanca
Wahid OAA, Mehana TA (2000) Impact of phosphate-solubilizing fungi on the yield and phosphorus-uptake by wheat and faba bean plants. Microbiol Res 155:221–227
Wakelin SA, Warren RA, Harvey PR, Ryder MH (2004) Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biol Fertil Soils 40:36–43
Acknowledgments
Dr. LMS Palni (Director GBPIHED) is thanked for encouragement and extending the facilities. The Ministry of Environment and Forests, and Department of Science and Technology, Govt. of India, New Delhi, are acknowledged for financial support.
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Rinu, K., Pandey, A. Temperature-dependent phosphate solubilization by cold- and pH-tolerant species of Aspergillus isolated from Himalayan soil. Mycoscience 51, 263–271 (2010). https://doi.org/10.1007/s10267-010-0036-9
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DOI: https://doi.org/10.1007/s10267-010-0036-9