Abstract
The gene lccK encoding a laccase of the white-rot basidiomycete Pleurotus ostreatus wild-type strain collected in Japan has been cloned, sequenced, and characterized. The isolated gene consists of 2929 bp with the coding region interrupted by 19 introns and flanked by an upstream region in which putative CAAT and TATA elements were identified. Two putative N-glycosylation sites and four putative copper-binding sites found in other fungal laccase are conserved in lccK. The cDNA contains an open reading frame of 1599 bp and the gene encodes 533 amino acids preceded by a signal peptide of 23 amino acids. The nucleotide sequence of the lccK cDNA showed high homology with those of laccases of other basidiomycetes.
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Received: August 22, 2002 / Accepted: October 9, 2002
Present address: Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo-nakano, Akita 010-0195, Japan
Correspondence to:K. Okamoto
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Okamoto, K., Ito, Y., Shigematsu, I. et al. Cloning and characterization of a laccase gene from the white-rot basidiomycete Pleurotus ostreatus . Mycoscience 44, 0011–0017 (2003). https://doi.org/10.1007/s10267-002-0075-y
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DOI: https://doi.org/10.1007/s10267-002-0075-y