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Effects of experimental periodontitis on the metabolic system in rats with diet-induced obesity (DIO): an analysis of serum biochemical parameters

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Abstract

Recent studies have shown that periodontitis accelerates the progression of obesity-associated metabolic diseases. Thus, we examined the influence of periodontitis on serum biochemical parameters of metabolic disease in a diet-induced obesity (DIO) rat. First, we established the DIO model using ten male rats fed with either basal diet (lean group) or high-fat diet (DIO group) for 12 weeks. Second, to examine the interaction between periodontitis and obesity, we divided 24 DIO rats into the following four groups. (1) Porphyromonas gingivalis (Pg) group was applied with Pg around the maxillary first molar (M1). (2) Ligature group was applied with ligature placement around M1. (3) Ligature/Pg group was treated with both ligature placement and Pg. (4) Control was non-treatment group. Serum biochemical parameters and maxillary histopathology were evaluated at 12 weeks. The DIO model demonstrated significant increases in body weight, serum insulin, alanine aminotransaminase (ALT) levels, and insulin resistance (HOMA-IR) compared to the lean group. In the DIO ligature and ligature/Pg groups, alveolar bone resorption and inflammatory cell infiltration were significantly increased compared to the control. Serum levels of fasting glucose, lactate dehydrogenase, and uric acid were also significantly higher, while the liver damage markers ALT and aspartate aminotransferase were only higher in ligature/Pg group. However, we observed no significant differences between the Pg group and Control. The present study suggested a possibility that periodontitis induced by ligature placement changed serum metabolic parameter regarding organs such as the liver in DIO rat.

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Acknowledgements

This study was carried out using the facilities and resources of The Nippon Dental University School of Life Dentistry at Tokyo. We are grateful to Dr. Toshiyuki Toen and Tetsuya Fukada for assistance with data acquisition and analysis.

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Authors and Affiliations

  1. Department of Life Science Dentistry, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, Tokyo, 102-8159, Japan

    Ryutaro Kuraji

  2. Department of Periodontology, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, Tokyo, 102-8159, Japan

    Ryutaro Kuraji, Miyako Fujita, Hiroshi Ito & Yukihiro Numabe

  3. The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, Tokyo, 102-8159, Japan

    Shuichi Hashimoto

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  1. Ryutaro Kuraji
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Correspondence to Ryutaro Kuraji.

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Kuraji, R., Fujita, M., Ito, H. et al. Effects of experimental periodontitis on the metabolic system in rats with diet-induced obesity (DIO): an analysis of serum biochemical parameters. Odontology 106, 162–170 (2018). https://doi.org/10.1007/s10266-017-0322-5

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